鱼藤酮长期作用对大鼠纹状体单胺递质、NO和NOS的影响

目的观察鱼藤酮长期作用对大鼠纹状体单胺递质和NOS/NO系统的变化规律.方法 Wistar大鼠每日皮下注射鱼藤酮90 d,于第30、60、90天处死动物,分离大鼠纹状体,分别以HPLC法和生化法测定其多巴胺(DA)、去甲肾上腺素(NA)、3,4-二羟基苯乙酸(DOPAC)、5-羟色胺(5-HT)、一氧化氮(NO)含量和一氧化氮合酶(NOS)活性.结果在鱼藤酮中毒各时相点,大鼠纹状体单胺递质均出现不同程度的变化,以DA、DOPAC、5-HT含量改变尤为明显;纹状体NO含量和NOS活性均明显升高,且中毒剂量越大、中毒时间越长NO、NOS升高越明显.结论纹状体单胺递质代谢紊乱和NOS/NO系统改变在鱼藤酮长期作用引起的中枢DA神经损伤中起重要作用.

Effects of long-term rotenone intoxication on the monoamine neurotransmitters, NO, and NOS in rat striatum

Objective To observe the changes of monoamines, nitric oxide (NO), and nitric oxide synthase (NOS) in rat striatum induced by long-term rotenone intoxication. Methods Wistar rats were daily administrated with rotenone at the doses of 0.335, 0.670, and 1.005 mg/kg (sc). Animals were sacrificed on days 30, 60 and 90. High performance liquid chromatography (HPLC) and photoelectric colorimetry were used to determine the concentrations of noradrenaline (NA), dopamine (DA), dihydroxyphenylaceticacid (DOPAC), 5-hydroxytryptamine (5-HT), NOS, and NO in the striatum, respectively. Results At each time point, rotenone induced significant changes of monoamines at various levels, especially the contents of DA, DOPAC, and 5-HT in the rat striatum. NO concentration and NOS activity in rat striatum increased remarkably in dose- and time-dependent manners. Conclusion The metabolic disturbance of monoamine neurotransmitters and NOS/ NO system in the rat striatum plays an important role in the central DA nerve injury induced by long-term rotenone intoxication.