葛根素在体外对成骨细胞增殖和分化的影响

背景：成骨细胞是骨代谢平衡过程中的关键功能细胞，植物雌激素对成骨细胞的增殖和分化有熏要影响，葛根素作为植物雌激素的一种，在体外以较大范围浓度对成骨细胞功能的影响仍少见报道。目的：观察葛根素在体外对大鼠成骨细胞增殖和分化功能的影响。方法：取新生Wistar大鼠的颅盖骨，对成骨细胞进行分离、培养、纯化及鉴定。将培养的成骨细胞随机分为对照组、10^-3-10-10mol／L不同浓度葛根素组，观察不同浓度葛根素对体外培养的成骨细胞增殖和碱性磷酸酶活性表达的影响。结果与结论：细胞经葛根素处理后10～-10-9gmol／L组成骨细胞增殖活性较对照组明显增加（P〈0．05），第3天增殖最快（P〈0．01），第4天开始下降；诱导第4天，各组碱性磷酸酶活性与对照组相比，差异均有显著性意义（P〈0．01），其中以10μmol／L组最显著（P〈0．01）。然而葛根素10μmol／L组成骨细胞增殖活性、碱性磷酸酶活性表达较对照组均减少（P〈0．05）。提示葛根素对成骨细胞的影响存在剂量依赖性，并且具有双向性，即在低浓度（105--10-8mol／L）下刺激骨形成：在高浓度（10^-3-10^-4mol／L）下抑制骨形成。

Effect of puerarin on proliferation and differentiation of osteoblasts in vitro

BACKGROUND： Osteoblasts play a key role in the process of bone metabolic balance. Phytoestrogen plays an important role in the proliferation and differentiation of osteoblasts. Puerarin acts as a kind of phytoestrogen, but the effect of puerarin with large range of concentrations on the function of osteoblasts in vitro is rarely reported. OBJECTIVE： To investigate the effect of puerarin on the proliferation and differentiation of osteoblasts in vitro METHODS： The crania of Wistar rats which were born within 24 hours were obtained in aseptic condition. After enzyme digestion, isolation, purification and identification of osteoblasts, the cultured osteoblasts were randomly divided into control group and experimental groups treated with 103 10-10mol/L puerarin. And then the effects of puerarin with various concentrations on proliferation of osteoblasts cultured in vitro and alkaline phosphatase activity were observed. RESULTS AND CONCLUSION： After treated with 10-5010-gmol/L puerarin, the proliferation of osteoblasts and the but it began to decrease at day 4; the alkaline phosphatase activity were significantly increased as compared with the control group （P 〈 0.05）, and the proliferation of osteoblasts was fastest at day 3 （P 〈 0.01 ）, but it began to decrease at day 4; the alkaline phosphatase activity between the control and experimental groups had no significant difference after inducement for 4 days （P 〈 0.01 ）. However, proliferation of osteoblasts and the alkaline phosphatase activity in the 10.3 mol/L puerarin group were decreased as compared with the control group （P 〈 0.05）. These results suggest that puerarin has a double-way effect on the in vitro osteoblasts in a dose-dependent manner. Low-close puerarin （10^-5-100-8 mol/L） can show positive functions on the forming of osteoblasts, but high-dose puerarin （10-3- 10^-4 mol/L） can inhibit bone formation.