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| 版纳小型猪脱细胞骨基质的抗原检测及力学性状 | |
| 引用本文： | 董世武,应大君,朱楚洪,孙建森,张伟,曾养志. 版纳小型猪脱细胞骨基质的抗原检测及力学性状[J]. 生物医学工程学杂志, 2006, 23(3): 551-555 |
| 作者姓名： | 董世武 应大君 朱楚洪 孙建森 张伟 曾养志 |
| 作者单位： | 1. 第三军医大学,解剖学教研室,国家教育部生物力学与组织工程重点实验室生物力学研究室,重庆,400038 2. 云南农业大学,动物科学与技术学院,昆明,650201 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 制备版纳小型猪脱细胞骨基质，进行组织学和生物力学观察，并进行了异种抗原a—gal表达的检测，结果表明经脱细胞处理后，去除了骨组织的细胞成分，同时消除了抗原性，力学性质上与正常对照无明显差异，可以作为一种合适的骨组织工程支架材料。 |
| 关 键 词： | 脱细胞骨基质 异种抗原 生物力学 |
| 收稿时间： | 2004-04-12 |
| 修稿时间： | 2004-04-122005-02-03 |
Antigen Measurement and Biomechanical Characteristics of the Inbred-line Banna Mini-pig Acellular Bone Matrix |
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| Dong Shiwu,Ying Dajun,Zhu Chuhong,Sun Jiansen,Zhang Wei,Zeng Yangzhi. Antigen Measurement and Biomechanical Characteristics of the Inbred-line Banna Mini-pig Acellular Bone Matrix[J]. Journal of biomedical engineering, 2006, 23(3): 551-555 | |
| Authors: | Dong Shiwu Ying Dajun Zhu Chuhong Sun Jiansen Zhang Wei Zeng Yangzhi |
| Affiliation: | 1.The Key Lab of Biomechanics under the Ministry of Education ,Department of Anatomy, The Third Military Medical University,Chongqing 400038,China; 2.School of Animal Science and Technology,Yunnan Agricultural University,Kunming 650201,China |
| Abstract: | In this study, we prepared the acellular bone matrix of the inbred-line Banna mini-pig by using tissue engineering method and evaluated its possible application in bone tissue engineering. Histological analysis, xenoantigen expression and biomechanical measurement were performed on the matrix. HE staining and scanning electron microscopy showed the cellular components were almost removed. Immunohischemical result demonstrated that the xenoantigen, a-gal,was also eliminated. There was no statistically significant difference between the acellular bone matrix group and control group. The acellular bone matrix can provide appropriate space structure and strength for grafts. In conclusion, our data suggest that acellular bone matrix is a new kind of ideal bone scaffold material. |
| Keywords: | Acellular bone matrix Xenoantigen Biomechanics |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |