山楂原花青素和葡萄籽原花青素活性比较

目的比较山楂原花青素(OPC)与葡萄籽OPC的生物活性。方法体外将肝癌细胞Hep-S、小鼠肉瘤S-180、小鼠胸腺、脾淋巴细胞、肾细胞和肝细胞分别与不同浓度的山楂OPC或葡萄籽OPC共同培养,用MTT法检测抑制Hep-S和S-180增殖的影响以及促进T细胞、B细胞、肾细胞和肝细胞增殖的影响。提取与山楂OPC或葡萄籽OPC共同培养的Hep-S细胞的DNA,检测Hep-S细胞的凋亡程度。结果山楂OPC比葡萄籽OPC抑制Hep-S和S-180增殖作用更好;其促进T细胞、B细胞、肾细胞和肝细胞增殖效果也更强;与葡萄籽OPC相比,山楂OPC使Hep-S细胞中的DNA发生更明显的凋亡,产生更多的DNA片段。结论山楂OPC具有比葡萄籽OPC更强的生物活性。

The comparative study of proanthocyanidins activity purified from hawthorn and grape seed

Purpose To compare proanthocyanidins bioactivity between hawthorn and grape seed.MethodsIn vitro Hep-S,S-180,mice thymus,splenic lymphocytes,renal cell and liver cell with different concentrations of hawthorn and grape seed proanthocyanidins were cultivated,detecting how hawthorn and grape seed proanthocyanidins inhibited proliferation of Hep-S and S-180 and promoted proliferation of T cells,B cells,renal cell and liver cell by the MTT method.Extracteding DNA of Hep-S cells which were cultivated with hawthorn and grape seed procyanidin,so as to detect Hep-S cell apoptosis.ResultsHawthorn proanthocyanidins has better inhibiting proliferation effect of Hep-S and S-180 than grape seed proanthocyanidins.It also has strong promoting proliferation effect of the thymus,splenic lymphocytes,renal cell and liver cell.Compared with grape seed proanthocyanidins,hawthorn proanthocyanidins make Hep-S cells produce more obvious DNA apoptosis and get more segment of DNA.ConclusionHawthorn proanthocyanidins have stronger biological activity than those of grape seed.