Struktur der Zellwandpolysaccharide in der Futtereiweiß-Hefe Candida spec. H. IV. Struktur der alkalilabilen Oligosaccharide im Mannan-Protein-Phosphat-Komplex

Mild alkaline degradation of the proteophosphomannan (PPM) under conditions that effect β-elimination of the residues, O-glycosidically bound on serine and threonine, release mainly mannose, glucose, mannobioses and mannotrioses with the same structures as those obtained by acetolysis of the polysaccharide component of the PPM. Methylation analysis and paper chromatography demonstrated the structural heterogeneity of the tetra-, penta- and hexasaccharide fractions containing 1,2- and 1,6-linked and 1,2,6-branched manno-oligosaccharides. Methylation analysis and acetolysis, paper chromatography and analysis of the carbohydrate composition of the oligosaccharide fractions DP 7–12 and DP 15–19 demonstrated inner core region like structures containing 1,2- and 1,6-linked mannose, 1,2,6- and 1,3,6-branchpoints and N-acetyl-d-glucosamine.