芪参益气浸膏对异丙肾上腺素致大鼠慢性心力衰竭治疗作用研究

目的:观察芪参益气浸膏对异丙肾上腺素(ISO)诱导大鼠慢性心力衰竭(CHF)的治疗作用和对心肌能量代谢的影响方法:142只雄性SD大鼠随机分为对照组和CHF组,模型制备采用皮下多点注射ISO法,4周后CHF组再随机分为模型组和芪参益气浸膏高、中、低3个治疗组及曲美他嗪治疗组.平均治疗5周后进行心脏超声检查,并测定心肌组织中高能磷酸化合物的含量和肌浆网Ca2 依赖型ATP酶(SERCA)活性.结果:芪参益气浸膏显著提高心衰大鼠左室短轴缩短率(FS%)(36.35±3.80vs 31.48±5.98,P＜0.05),左室射血分数也有改善趋势,心肌ATP和总腺苷(TAN)含量比模型组分别提高了19%和31%,与正常对照组无统计学差异(P＞0.05).芪参益气浸膏组和曲美他嗪组心肌SERCA活性显著高于模型组.结论:芪参益气浸膏有改善ISO诱导慢性心衰大鼠心功能的趋势,这种作用可能与心肌能量代谢的改善有关.

The protective effects of QiShenYiQiJinGao on isoproterenol-induced heart failure in SD rats

Objective:To investigate the protective effects of QiShenYiQiJinGao on isoproterenol-induced chronic heart failure(CHF) and its effects on the myocardial energy metabolism in SD rats. Methods:142 male SD rats were randomly divided into two groups:control group and CHF group. Rats in CHF group were injected subcutaneously with isopreteronol(ISO). Four weeks later,the rats in CHF group were randomly re-divided into five groups:CHF model group,trimetazidine treated group,QiShenYiQiJinGao high,middle and low dose group. After an average treatment for five weeks,echocardiogram was performed to assess the cardiac function changes of these rats. The myocardial high energy phosphates and sarcoplasmic reticulum Ca2+-ATPase(SERCA) activity were determined by enzymatic analyses. Results:Compare with the model group,FS was significantly increased(31.48 ± 5.98 vs 36.35 ± 3.80,P < 0.05) in QiShenYiQiJinGao group. Meanwhile,EF also had a trend to be improved in QiShenYiQiJinGao group. Furthermore,the level of ATP and TAN(TAN=ATP+ADP+AMP) was increased by 19% and 31%,which was similar to the control group(P > 0.05). Compare with the model group,the SERCA activity was significantly increased in QiShenYiQiJinGao and trimetazidine treated group. Conclusion:QiShenYiQiJinGao could improve the cardiac function of ISO-induced CHF in SD rats via enhancing myocardial energy metabolism.