苯扎贝特对血小板源生长因子诱导的血管平滑肌细胞增殖和小凹蛋白1表达的影响

目的 观察苯扎贝特对血小板源生长因子诱导的大鼠血管平滑肌细胞增殖及小凹蛋白1表达的影响并探讨其机制.方法 体外植块贴壁法培养大鼠胸主动脉血管平滑肌细胞,噻唑兰比色法检测各组细胞增殖情况,Western Blotting法观察各组小凹蛋白1的表达情况.结果 不同浓度的苯扎贝特对血小板源生长因子诱导的血管平滑肌细胞增殖均有一定的抑制作用,随浓度增加,其抑制作用增强(P<0.01).血小板源生长因子干预组小凹蛋白1的表达较对照组明显降低(P<0.01),而用不同浓度的苯扎贝特干预后小凹蛋白1的表达明显升高(P<0.01),且随着苯扎贝特浓度增加,小凹蛋白1的表述明显增加.结论 苯扎贝特抑制血小板源生长因子诱导的血管平滑肌细胞增殖的同时可以上调小凹蛋白1的表述.

Effects of Bezafibrate on the Expression of Caveolin-1 and the Proliferation of Smooth Muscle Cells Induced by Platelet-Derived Growth Factor

Aim To observe the effects of bezafibrate on the expression of caveolin-1 and the proliferation of vas-cular smooth muscle cells (VSMC) induced by platelet-derived growth factor (PDGF) and investigate the mechanism of it. Methods VSMC were cultured in vitro, the proliferation of VSMC were obtained through thiazolyl blue colorimetric technique (MTT). The expression of caveolin-1 was assayed by Western Blotting. Results Compared with the con-trol group, PDGF could increasingly induce the proliferation of VSMC (P < 0.01). Bezafibrate inhibited the proliferation of VSMC induced by PDGF in concentration-dependent manner (P < 0. 01). Compared with the control group, PDGF could decrease caveolin-1 protein expression (P < 0. 01). Bezafibrate increase the expression of caveolin-1 compared with the PDGF group(P < 0.01). With the increase of Bezafibrate's concentration, the expression of caveolin-1 could in-crease accordingly. Conclusion Bezafibrate can decrease the proliferation of VSMC induced by PDGF and increase the expression of caveolin-1 protein.