荧光原位杂交技术检测c-erbB-2和p53基因在胃癌中的表达

目的：探讨胃癌组织中ｃ－ｅｒｂＢ－２和Ｐ５３基因的表达及其意义。方法：应用荧光原位杂交技术对５５例有癌及移行区、正常区组织的常规石蜡包埋标本进行检测。结果：ｃ－ｅｒｂＢ－２和Ｐ５３基石 阳性率分别为３６．３６％和４５．４５％,移行芡为１０．９１％和３．６４％,正常粘膜区为３．６５％和０,三区间差异无有极显著意义（Ｐ值均〈０．０１）。在肠型和弥温型胃癌中,ｃ－ｅｒｂＢ阳性率分别为５１．６１％和１６．

Detection of c-erbB-2 and p53 gene expression in human gastric carcinoma by fluorescence in situ hybridization

Objective:To investigate the expression of c erbB 2 and p53 gene in gastric carcinoma Method:The C erbB 2 and p53 expressions were dedected by in situ hybridization (FISH) for 55 cases of gastric carcinoma tissues, their transitional region tissues and normal tissues of formalin fixed, paraffinembedded sections Results:The positive rate of c erbB 2 and p53 were 36 1%and 45 5%in cancer tissues, 10 91%and 3 64%in transitional regions, 3 64%and 0 in normal regions respectively There were highly significant differences among the three regions (all P< 0 001) In intestinal type and diffuse type gastric carcinoma, the incidences of c erbB 2 were 51 61%and 16 37%, and of p53 were 25 8%and 70 83%respectively,which was significantly different between two types (all P< 0 05) The expression of c erbB 2 and p53 gene were also correlated with the histological grades of the tumors (P< 0 01 or 0 05) The positive of C erbB 2 gene was related to lymph node metastasis of gastric carcinoma (P< 0 05) Conclusions:The amplification of c erbB 2 gene and mutation of p53 are related to genesis type, differentiation and metastasis, and thus the detection of these gene expression might be useful in evaluating the biological behavior of the tumors