C—myc蛋白在乳腺良恶性病变的表达及与DNA含量的关系

目的：检测乳腺良恶性病变癌ｃ－ｍｙｃ表达及与ＤＮＡ倍体的关系。方法：利用免疫组化（ＬＳＡＢ）法和图像分析技术对乳腺癌８２例和乳腺良性病变２２例进行ｃ－ｍｙｃ蛋白免疫组化检测。结果：ｃ－ｍｙｃ蛋白免疫组化染色的着色面积与着色深度高度相关（Ｐ＜０．０１），着色深度和着色面积又与平均灰度、平均光密度及平均积分光密度高度相关。ｃ－ｍｙｃ癌基因蛋白在乳腺良恶性病变表达率差别无统计学意义（Ｐ＜０．０５）。但良性病变ｃ－ｍｙｃ染色多呈轻度阳性，少数呈中到强度阳性，而且着色部位多以胞核胞质均着染色。恶性肿瘤ｃ－ｍｙｃ免疫组化染色多呈中到强度阳性，少数呈弱阴性，且以胞质着色为主，二者之间差别有显著意义（Ｐ＜０．０５）。结论：乳腺良恶性病变都可有ｃ－ｍｙｃ激活，ｃ－ｍｙｃ与细胞增殖有关，并非恶性肿瘤所特有。但结合着色程度及着色部位对良恶性病变的鉴别仍然有意义。

THE EXPRESSION OF C-myc ONCOPROTEIN IN BREAST BENIGN AND MALIGNANT LESIONS AND ITS CORRELATION WITH DNA PLOIDS

Objective:To study the expression of c-myc oncogene protein and its correlation with DNA ploid. Mothods:22 cases of benign and 82 cases of malignant were measured by immunohistochemistry and Feulgen stain. Results: There existed high correlation between the percentage of positive cells and stain intensity of c-myc( P <0.01).Their stain intensity and percentage of positive cells were correlated again with the average grey level and light density level( P <0.01).There was no significant difference between benign and malignant lesions. But benign were cytoplasm and nucleus stained equally and weekly. Breast carcinoma were mainly strong cytoplasmic stain. There existed significant differences of stain intensity and location between these two groups( P <0.05).Conclusion: c-myc oncogene products were expressed in both of two groups, there is still a role in diagnosis of breast malignant and benign lesions by combining staining intensity and locations.