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| 毛细管电泳法分析糖基化血红蛋白 | |
| 引用本文： | 苗杰,府伟灵,邱振薇,李学慧,王景胜. 毛细管电泳法分析糖基化血红蛋白[J]. 国际检验医学杂志, 2014, 0(14): 109-110,1922 |
| 作者姓名： | 苗杰 府伟灵 邱振薇 李学慧 王景胜 |
| 作者单位： | 苗杰 (解放军第四0四医院检验科,山东威海,264200)； 府伟灵 (第三军医大学西南医院检验科,重庆,400038)； 邱振薇(海军威海水警区后勤部卫生科,山东威海,264200)； 李学慧 (解放军第四0四医院检验科,山东威海,264200)； 王景胜 (解放军第四0四医院检验科,山东威海,264200)； |
| 摘 要： | 目的：探讨毛细管电泳在分析糖基化血红蛋白水平方面的临床应用价值。方法该文利用长30 cm、内径25μm 的未涂层熔融石英毛细管，利用血红蛋白在415 nm 波长处的特异性吸收峰，二极管阵列（PDA）法检测健康人与糖尿病及高危人群中的糖基化血红蛋白（HbA1c）水平。在研究过程中，对精密度和准确度进行了精确分析。结果 HbAlc 和非 HbAlc 成功分离，峰型清晰锐利且能对 HbAlc 进行定量分析。结论毛细管电泳法具有特异性好、精密度和准确度高等优点，因此，该法应该在临床上被推广应用。 |
| 关 键 词： | 毛细管电泳法 糖化血红蛋白 检测 |
Analysis of glycosylation hemoglobin by capillary electrophoresis |
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| Affiliation: | Miao Jie, Fu Weiling, Qiu Zhenwei, Li Xuehui, Wang Jingsheng ( 1. Department of Clinical Laboratory, 404 Hospital of PLA ,Weihai, Shandong 264200, China 2. Department of Laboratory Medicine, Southwest Hospital, Third Military Medical University, Chongqing 400038, China 3. Section of Health, Department of Logistics, Weihai Coastal De fence Regions, Weihai , Shandong 264200, China) |
| Abstract: | Objective To discuss the application value of the capillary electrophoresis in detecting the glycosylation hemoglobin （HbA1c）level.Methods By utilizing the uncoated fused silica capillary column（30 cm in length and 25 μm in diameter）and the specific adsorption peak,the HbA1c levels in the healthy people,patients with diabetes mellitus（DM）and high risk people were de-tected by the photodiode array（PDA）method at the wavelength of 415 nm.During the research process,the precision and accuracy were perfomred the accuracy analysis.Results HbA1c could be effectively separated from non-HbA1c,with a sharp peak.And the relative quantity of HbA1c could be determined.Conclusion The capillary electrophoresis is characterized by specificity,high precision,and high accuracy.Therefore,it should be widely applied in clinic. |
| Keywords: | capillary electrophoresis glycosylation hemoglobin detection |
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