声门上型喉癌组织中肿瘤相关巨噬细胞浸润与微淋巴管生成关系

目的 研究声门上型喉肿瘤癌内及癌周肿瘤相关巨噬细胞(TAM)计数与微淋巴管生成的关系。方法 通过免疫组化法测定84例声门上型喉癌癌内及癌周标本微淋巴管密度（LMVD)及TAM的表达，并分析癌内及癌周LMVD、TAM计数与声门上型喉癌临床病理学因素及生存率的关系。结果 TAM浸润与肿瘤微淋巴管生成呈正相关，癌内(r=0.253, P=0.02)，癌周(r=0.270, P=0.013)。癌内TAM浸润随肿瘤T分期上升而增加，癌内TAM浸润越多肿瘤预后越差，淋巴结转移组的癌周TAMs显著高于淋巴结无转移组，发生远处转移组的癌内及癌周LMVD显著高于未发生远处转移组，癌周LMVD越高肿瘤预后越差。Kaplan-Meier生存分析显示癌内高密度TAM及高密度微淋巴管组5年生存率(47.6% )显著低于低密度组(86.4%），癌周高密度TAM及高密度微淋巴管组5年生存率(50.7% )显著低于低密度组(81.0%)(P＜0.05)。结论 TAM和微淋巴管生成与声门上型喉癌转移和不良预后密切相关。

The relationship between rumor-associated macrophage infiltration and lymphangiogenes in supraglottic laryngeal carcinoma

Objective To study the correlation between intratumoral and peritumoral tumor associated macrophage(TAM) infiltration and lymphatic microvessel density (LMVD) in supraglottic laryngeal carcinoma．Methods Immunohistochemistry was used to detect CD68 and podoplanin's expression in intratumoral and peritumoral areas of 84 specimens of supraglottic laryngeal carcinoma and the associations between LMVD、TAM count and clinicopathological features, and survival rate of supraglottic laryngeal carcinoma were analyzed．Results TAM infiltration showed a significant positive connection with lymphangiogenesis in the intratumoral (r=0.253, P=0.02) and peritumoral areas(r=0.270, P=0.013)．Patients with high intratumoral TAM count showed a higher T staging and demonstrated a poorer outcome. The peritumoral TAM count of patients with lymph node metastasis were significantly higher than that of patients without lymph node metastasis. Patients with high intratumoral or peritumoral LMVD suffering from remote metastasis were more numerous than those with low intratumoral or peritumoral LMVD.Patients with high peritumoral LMVD had a poorer outcome, the difference was significant（P<0.05）. Kaplan-Meier survival analysis showed the 5-yearsurvival rate of high intratumoral TAM and LMVD (47.6%)was significantly lower than the contrary(86.4%）. Also, the 5-year survival rate of high peritumoral TAM and LMVD (50.7%)was significantly lower than the low group(81.0%,P<0.05). Conclusions TAM and lymphangiogenesis are associated with metastases and poor prognosis of supraglottic laryngeal carcinoma.