酪蛋白磷酸多肽钙氟磷复合体对牙釉质缺损再矿化的实验研究

目的　探讨 2 %酪蛋白磷酸多肽钙氟磷复合体 (caseinphosphopeptide amorphouscalciumfluoridephosphatecomplexes,CPP ACFP)溶液对人工牙釉质表层下缺损的再矿化作用。方法　从人第三磨牙切取 2 8块釉质片 (每片制备两条表层下釉质缺损 ) ,随机平分为 4组 ,每组分别于再矿化液中浸泡1、3、5、10d。将再矿化后的标本切片 ,显微放射照相 ,测定矿物质含量。结果　经 1、3、5和 10d再矿化后 ,矿化液分别取代人工牙釉质缺损丢失矿物质的 9 19%、14 2 7%、2 9 0 7%和 38 45 % (y =8 9316x0 63 47,R2 =0 932 2 ;y :再矿化率 ,x :时间 )。经One wayScheffe差异多因素分析比较发现 :每两组间再矿化率差异具有显著性。结论　实验室中CPP ACFP对人工牙釉质缺损有明显的再矿化作用

The remineralization of enamel lesions by casein phosphopeptide-amorphous calcium fluoride phosphate in vitro

Objective To evaluate the remineralizing effect on artificial enamel subsurface lesions in vitro by 2% casein phosphopeptide amorphous calcium fluoride phosphate complexes (CPP ACFP) solution. Methods Twenty eight enamel blocks were cut from human third molars, in which 2 lesions were prepared from each block. The 28 samples were randomly assigned to the four study groups, in which each group was incubated in remineralization solution for 1, 3, 5, 10 days respectively. After remineralization the samples were sectioned, microradiographied and the mineral content were determined by microdensitometry. Results The remineralizing solution replaced 9 19%, 14 27%,29 07%,38 45% of mineral lost after 1, 3, 5, 10 days respectively (y= 8 931 6 x 0 6347 , R 2= 0 932 2; y: remineralizing rate, x: time). Significant differences were found in remineralizing rate between any two groups using One way Scheffe multiple comparison. Conclusions The 2% CPP ACFP solution could significantly remineralize the artificial enamel subsurface lesion in vitro. The remineralizing rates were increased with the experiment time (within 10 days).