咔唑法同时测定玻璃酸钠和硫酸软骨素的含量

目的：建立咔唑法同时测定玻璃酸钠（SH）和硫酸软骨素（CS）含量的方法。方法：通过醋酸钠调节样品的酸碱度和离子强度，氯化十六烷基吡啶（CPC）可选择性地沉淀CS，而SH不沉淀，从而将SH和CS进行分离。结果：样品预处理条件为：精密量取SH和CS的混合溶液10mL，加入醋酸钠1．6g，12．5mmot／LCPC10mL，充分搅拌后，静置1h，20μm滤膜过滤；滤液用于测定SH的含量；滤饼经1．4mol／L氯化钠溶液超声解离后，用于测定CS的含量；SH和CS的平均回收率分别为101．2％和99．4％，RSD分别为1．05％和0．96％。结论：本法可同时测定SH和CS的含量，相互之间无干扰。

Carbazole method for simultaneous determination of sodium hyaluronate and chondroitin sulfate

Objective ：To establish a carbazole method for the simultaneous determination of sodium hyaluronate （SH） and chon- droitin sulfate （CS）. Methods： By regulating acidity or alkalinity and ionic strength with sodium acetate,CS was selectively precipita- ted with cetylpyridinium chloride （CPC） ,whereas SH was not. So SH and CS were separated. Results：The sample pretreatment condi- tions were as follows：Measuring 10 mL mixture of SH and CS accurately,adding 1.6 g sodium acetate and 10 mL CPC of 12.5 mmol/ L, standing for 1 h after stirring thoroughly, then, the solution was filtered with 20 μm filter membrane and the filtrate was used to deter- mine the content of SH ;the precipitates on the filter membrane were resolved with 1.4 mol/L NaC1 solution by ultrasonic treatment for the determination of CS. The average recoveries of SH and CS were 101.2% and 99.4% with RSD of 1.05% and 0.96% ,respectively. Conclusion ：This method can be used for the simultaneous determination of SH and CS without interference with each other.