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| GABA对大鼠垂体前叶细胞离体增殖的影响及其A受体亚单位mRNA的表达 | |
| 引用本文： | 朱运龙,张万会,王复周,张建华,胡玉珍,陈健康,梅琦,赵超. GABA对大鼠垂体前叶细胞离体增殖的影响及其A受体亚单位mRNA的表达[J]. 神经解剖学杂志, 1994, 0(4) |
| 作者姓名： | 朱运龙 张万会 王复周 张建华 胡玉珍 陈健康 梅琦 赵超 |
| 作者单位： | 第四军医大学生理学教研室 |
| 摘 要： | 本实验利用原代细胞培养体系，观察到ＧＡＢＡ对大鼠垂体前叶细胞的氚标胸苷（３Ｈ－ＴｄＲ）基础掺入率有明显的抑制性效应，且可被印防己毒素所反转。培养细胞的原位杂交实验表明，ＧＡＢＡ_Ａ受体的β_２亚单位ｍＲＮＡ可表达于多数垂体前叶细胞中，提示ＧＡＤＡ的上述作用可能系ＧＡＢＡ_Ａ型受体介导的。 |
| 关 键 词： | GABA，垂体前叶，细胞增殖，原位杂交 |
ACTIONS OF GABA ON THE PROLIFERATION OF CULTURED RAT ANTERIOR PITUITARY CELLS WITH REFERENCE TO IT’S TYPE A RECEPTOR SUBUNIT mRNA EXPRESSION |
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| Zhu Yunlong,Zhong Wanhui,Wang Fuzhou,Zhang Jianghua,Hu Yuzhen,Chen Jiangkang,Mei Qi,Zhao Chao. ACTIONS OF GABA ON THE PROLIFERATION OF CULTURED RAT ANTERIOR PITUITARY CELLS WITH REFERENCE TO IT’S TYPE A RECEPTOR SUBUNIT mRNA EXPRESSION[J]. Chinese Journal of Neuroanatomy, 1994, 0(4) | |
| Authors: | Zhu Yunlong Zhong Wanhui Wang Fuzhou Zhang Jianghua Hu Yuzhen Chen Jiangkang Mei Qi Zhao Chao |
| Abstract: | This paper describes an inhibitory effert of GABA on the incorporation of￣3H-TdR into rat anterior pituitary cells in static primary monolayer cultures. The action was antagonizable by picrotoxin. In situ hybridization histochemiarry of cultured cells revealed a predominant expression of mRNA encoding β_2 subunit of GABA_A receptor in most of rat anterior pituitary cells, thus suggesting a possible mediation of the above effert by GABA_A receptors. |
| Keywords: | GABA(gamma-aminobutyric acid) antetior pituitary cell proliferation in situ hybridization histochemistry |
| 本文献已被 CNKI 等数据库收录！ | |