RP-HPLC法测定妇血康颗粒中原儿茶酸与原儿茶醛的含量

目的:建立以高效液相色谱法测定妇血康颗粒中原儿茶酸及原儿茶醛含量的方法。方法:色谱柱为Achirom Bond-1 C18(250mm×4.6mm,5μm),流动相为甲醇-水(20∶80,用冰醋酸调pH2.80),流速为1.0mL.min-1,检测波长为256nm(原儿茶酸)和280nm(原儿茶醛)。结果:原儿茶酸与原儿茶醛的检测浓度分别在5.49~73.22μg.mL-1(r=0.9999)与4.81~64.13μg.mL-1(r=0.9999)范围内与各自的峰面积积分值呈良好线性关系;二者平均回收率分别为96.86%和97.55%,RSD分别为2.52%(n=9)和3.82%(n=9)。结论:本方法简单、灵敏度高、重现性好,可用于妇血康颗粒的质量控制。

Determination of Protocatechuic Acid and Protocatechuic Aldehyde in Fuxuekang Granules by RP-HPLC

OBJECTIVE： To establish a RP- HPLC method for the simultaneous determination of Protocatechuic acid and Protocatechuic aldehyde in Fuxuekang granules. METHODS： The chromatography was carried on Achirom Bond- 1 C18 col- umn（250 mm × 4.6 mm, 5μm） . The mobile phase was composed of methanol - water （20 ： 80, pH = 2.80 adjusted by glacial acetic acid） at a flow rate of 1.0 mL · min ^-1. The detection wavelengths were programmed at 256 nm for Protocatechuic acid and at 280 nm for Protocatechuic aldehyde. RESULTS： The linear ranges for Protocatechuic acid and Protocatechuic aldehyde were 5.49-73.22 μg · mL^-1（ r = 0.999 9） and 4.81-64.13μg · mL ^-1（ r= 0.999 9）, respectively, and their average recoveries were 96.86% （RSD = 2.52%, n = 9） and 97.55% （RSD = 3.82%, n = 9） respectively. CONCLUSION： The method is simple, sensitive, and reproducible, and it is applicable for the quality control of Fuxuekang granules.