纳米硫化铅对大鼠海马及皮质氧化损伤作用的研究

目的探讨纳米硫化铅对大鼠海马及皮质的氧化损伤作用。方法清洁级SD雄性大鼠60只,随机分为3组,即对照组、纳米硫化铅低、高剂量组,低、高剂量组分别气管灌注15、30mg/kg粒径20nm的硫化铅混悬液1ml,每周1次,连续染毒3个月后测定3个组大鼠体质量变化;检测大鼠血清、海马及皮质中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活力和丙二醛(MDA)含量。结果染毒组大鼠血清、海马及皮质中SOD、GSH-Px的活力及MDA含量分别与对照组比较,差异均有统计学意义(P<0.05)。染毒组大鼠血清、海马及皮质中SOD、GSH-Px活力均降低,MDA含量增高。结论纳米硫化铅可能引起海马及皮质中SOD、GSH-Px活力降低,MDA含量增加,可导致海马及皮质抗氧化能力减弱。

Study of oxidative damage induced by nano lead sulfide on hippocampus and cortex of rats

Objective To explore the oxidative damage induced by nano lead sulfide(PbS)on hippocampus and cortex of rats.Methods 60 male SD rats were randomly divided into 3 groups,i.e.the control group,low and high dosages nano lead sulfide groups.The rats in low and high exposed groups were instilled intratracheally with 1 ml nano sized(particulate diameter 20 nm)lead sulfide suspension of 15 and 30 mg/kg respectively once a week consecutively for 3 months.After instillation the mass of each rat in all groups was weighed to see the changes.The activities of SOD and GSH-Px and contents of MDA were measured.Results The activities of SOD and GSH-Px and contents of MDA in the rat serum of both exposed groups were compared with those of the controls respectively,the differences were significant(P<0.05).All the activities of SOD and GSH-Px in rat serum,hippocampus and cortex of the exposed groups lowered,and MDA contents increased.Conclusions The results show that nano PbS may induce the activities of SOD and GSH-Px of hippocampus and cortex to reduce,and the contents of MDA to increase.It suggests that the antioxidant capacity of hippocampus and cortex might be weakened.