定量PCR检测乙型肝炎患者血清HBV DNA及其临床意义

目的:了解不同临床类型乙型肝炎患者HBV DNA含量并探讨其临床意义。方法:应用荧光定量聚合酶链反应(FQ-PCR)技术检测163例乙型肝炎患者血清HBV DNA含量,应用ELISA法检测上述患者HBV感染血清免疫学标志物(HBV-M),并对两者进行比较。结果:血清HBV DNA水平在不同乙型肝炎临床类型中无显著性差异(P>0.05);与HBV-M对比,HBsAg、HBeAg、抗-HBc阳性病人的检出率为87.76％,HBsAg、抗-HBe、抗HBc病人的检出率为45.71％,HBsAg、抗-HBc病人的检出率为42.86％,抗-HBc单项阳性病人的检出率亦达40.00％;血清HBVDNA含量与黄疸的程度及转氨酶水平的高低未见相关。结论:提示HBV DNA的复制状态与不同乙型肝炎临床类型无明显相关关系;HBV DNA含量高低与肝功能受损程度亦无相关关系。

Detection of Serum HBV DNA in Patients with Hepatitis B by Quantitative PCR and It''''s Significance

Objective To investigate the HBV DNA level in serum of miscellaneous patients suffering from hepatitis. Method: The HBV DNA in sera of patients suffering from hepatitis B was determined by quantitative PCR assay. And HBV-M by ELISA assay. Results: No significant variation of HBV DNA concentration in miscellaneous patients existed (P>0. 05). The concentration in HBsAg , HBeAg , HBcAb group with a positive rate of 87. 76%, in HBsAg, HBeAb , HBcAb group the mount was 4. 71%, in HBsAg , HBcAb group the mount was 42. 86%, in HBcAb group the mount was 40. 00%. The HBV DNA concentration were not related with ALT levels and jaundice degree. Conclusion: The levels of viral replication were not correlated with the different clinical type of heptitis ;the concentration of HBV DNA in serum was not related with liver injures.