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Epstein-Barr virus DNA in Reed-Sternberg cells of Hodgkin''s disease is frequently associated with CR2 (EBV receptor) expression
Authors:N.M. JIWA  P. VAN DER VALK  H. MULLINK  W. VOS  A. HORSTMAN  M.M. MAURICE  D.E.M. OLDE-WEGHUIS  J.M.M. WALBOOMERS  C.J.L.M. MEIJER
Affiliation:Department of Pathology, Free University Hospital, Amsterdam, The Netherlands.
Abstract:We studied 44 cases of Hodgkin's disease for the presence of Epstein-Barr virus (EBV) DNA, its localization and the expression of the EBV receptor on the tumour cells. EBV DNA was found in 52% (16/31) of the Hodgkin's lymphomas using the polymerase chain reaction. With a very sensitive non-radioactive DNA in situ hybridization technique in combination with immunohistochemistry for CD 30 or CD 15 antigens, EBV DNA was localized to Reed-Sternberg cells and its mononuclear variants. The relationship between the presence of EBV DNA and the expression of the EBV-receptor CR2 (CD 21) on Reed-Sternberg cells was studied using the same techniques and two different monoclonal anti-CD 21 antibodies. CR2 could be detected on a substantial number of the Reed-Sternberg cells in EBV DNA positive Hodgkin's lymphomas (9/12; 75%), whereas in EBV negative cases positivity with anti-CD 21 was rare (1/13; 8%). The results indicate that CR2 expression on Reed-Sternberg cells and the presence of EBV DNA sequences are frequently associated in Hodgkin's lymphomas.
Keywords:keywords    Epstein-Barr virus    Hodgkin's disease    Epstein-Barr virus receptor    DNA in situ hybridization    immunohistochemistry
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