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Determination of vinca alkaloids in human plasma by liquid chromatography/atmospheric pressure chemical ionization mass spectrometry |
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| Authors: | Jacqueline Ramírez Kenneth Ogan M. J. Ratain |
| Affiliation: | (1) Section of Hematology/Oncology, Department of Medicine, The University of Chicago Medical Center, MC 2115, 5841 S. Maryland Ave., Chicago, IL 60637, USA Tel. (312)702-1815; Fax (312)702-2770, US;(2) Hitachi Instruments, Inc., 44 Old Ridgebury Road, Danbury, CT 06810, USA, US;(3) Committee on Clinical Pharmacology, The University of Chicago, MC 2115, 5841 S. Maryland Ave., Chicago, IL 60637, USA, US;(4) Cancer Research Center, The University of Chicago, MC 2115, 5841 S. Maryland Ave., Chicago, IL 60637, USA, US |
| Abstract: | A sensitive assay was developed for the quantitation of vinblastine, desacetylvinblastine and vincristine using liquid chromatography-atmospheric pressure chemical ionization mass spectrometry (LC-APCI-MS). Analyses were performed on an Ultrasphere C18 microbore column using ammonium acetate as mobile phase. The calibration curves were linear across the range of 0.51–4.00 ng/ml (0.63–4.93 nM) for vinblastine, 0.74–3.93 ng/ml (0.96–5.11 nM) for desacetylvinblastine and 0.30–3.95 ng/ml (0.36–4.79 nM) for vincristine. Vinca alkaloid concentrations were measured with an accuracy and precision within 11%. This assay could be implemented to determine the plasma concentrations for pharmacokinetic studies of vinblastine, desacetylvinblastine and vincristine in conjunction with clinical trials. Received: 15 December 1995 / Accepted: 16 June 1996 |
| Keywords: | Vinblastine Desacetylvinblastine Vincristine Liquid chromatography-atmospheric pressure Chemical ionization mass spectrometry |
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