| 复方天葡片抑制H2O2诱导小鼠淋巴细胞凋亡的机制研究 |
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| 引用本文： | 魏日胞,王永新,曹丽,卓莉,傅博,李平.复方天葡片抑制H2O2诱导小鼠淋巴细胞凋亡的机制研究[J].中国实验血液学杂志,2011,19(1):211-214. |
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| 作者姓名： | 魏日胞 王永新 曹丽 卓莉 傅博 李平 |
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| 作者单位： | 1. 解放军总医院肾科、全军肾脏病研究所暨重点实验室,北京,100853
2. 空军航空医学研究所,北京,100142 |
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| 基金项目： | 国家科技重大专项(2010ZX9102-204); 国家自然科学基金(81072914); 全军医药卫生科研基金课题(06MA265); 军队中医药专项课题(10ZYZ255) |
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| 摘 要： | 本研究旨在探讨复方天葡片(tianpupian,TPP)抑制过氧化氢(H2O2)诱导淋巴细胞凋亡的作用机制。采用MTT法观察TPP对H2O2导致的淋巴细胞死亡率的影响;应用流式细胞仪AnnexinV/PI双染法、JC-I线粒体膜电位及caspase3酶活性的荧光化学发光法检测TPP对H2O2诱导淋巴细胞凋亡的抑制作用。结果表明:TPP通过拮抗H2O2导致的线粒体膜电位下降,抑制caspase3酶活性而降低H2O2诱导的淋巴细胞凋亡率。结论TPP对H2O2诱导的淋巴细胞凋亡具有显著的抑制作用,其机制与线粒体膜电位下降及caspase3酶活性抑制有关。
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| 关 键 词： | 复方天葡片 过氧化氢 淋巴细胞 细胞凋亡 |
Protective Effect of Compound Tianpupian(TPP)against H2O2-induced Apoptosis of Murine Splenic Lymphocytes |
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| WEI Ri-Bao,WANG Yong-Xin,CAO Li,ZHUO Li,FU Bo,LI Ping.Protective Effect of Compound Tianpupian(TPP)against H2O2-induced Apoptosis of Murine Splenic Lymphocytes[J].Journal of Experimental Hematology,2011,19(1):211-214. |
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| Authors: | WEI Ri-Bao WANG Yong-Xin CAO Li ZHUO Li FU Bo LI Ping |
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| Institution: | WEI Ri-Bao,WANG Yong-Xin1,CAO Li1,ZHUO Li,FU Bo,LI Ping Department of Nephrology,Kidney Center & Key Laboratory,Chinese PLA General Hospital,Beijing 100853,China,1Institute of Aviation Medicine,Chinese Air Force,Beijing 100142 |
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| Abstract: | The aim of this study was to explore the protective effect of compound tianpupian(TPP) against H2O2-induced the apoptosis of murine splenic lymphocytes and its machanism.The cell apoptosis rate was detected by MTT method;the cell apoptosis and mitochondrial membrance potential were detected by flow cytometry(FCM) with Annexi-V/ PI double staining and JC-1 staning method,respectively;and caspase 3 relative activity was determined by colorimetry.The results indicated that after treating with H2O2,the absorban... |
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| Keywords: | compound tianpupian hydrogen peroxide lymphocyte apoptosis |
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