| 小檗碱对3T3-L1脂肪细胞脂联素表达的影响 |
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| 引用本文： | 谷卫,曾文衡,胡海英.小檗碱对3T3-L1脂肪细胞脂联素表达的影响[J].中国中药杂志,2005,30(4):286-288. |
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| 作者姓名： | 谷卫 曾文衡 胡海英 |
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| 作者单位： | 浙江大学,医学院,附属第二医院,浙江,杭州,310009 |
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| 摘 要： | 目的:探讨小檗碱和胰岛素对3T3-L1脂肪细胞脂联素表达的影响。方法:检测分别经小檗碱及胰岛素处理后3T3 L1脂肪细胞脂联素表达水平改变,以β actin为内对照,半定量法RT PCR测定脂联素mRNA表达。结果:经小檗碱(10μmol·L-1)干预后3T3 L1脂肪细胞脂联素表达显著增加(P<0.05),加入胰岛素后脂联素的表达受到抑制,高浓度胰岛素有显著抑制作用(P<0.05)。结论:体外实验中,小檗碱使3T3-L1脂肪细胞脂联素的表达增加,而胰岛素可抑制小檗碱增加脂联素表达的作用。
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| 关 键 词： | 小檗碱 脂联素 胰岛素 3T3L1脂肪细胞 |
| 文章编号： | 1001-5302(2005)04-0286-03 |
| 收稿时间： | 2004/5/27 0:00:00 |
Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte |
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| GU Wei;ZENG Wen-heng;HU Hai-ying.Effects of berberine on adiponectin mRNA expression in 3T3-L1 adipocyte[J].China Journal of Chinese Materia Medica,2005,30(4):286-288. |
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| Authors: | GU Wei;ZENG Wen-heng;HU Hai-ying |
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| Institution: | The 2nd Affiliated Hospital, Medical College, Zhejiang University, Hangzhou 310009, China. guwei@aiocrm.com |
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| Abstract: | OBJECTIVE: To explore the effects of berberine and insulin on adiponectin mRNA expression in 3T3-L1 adipocyte. METHOD: The 3T3-L1 adipocyte was treated with berberine and insulin for 48 hours, the level of adiponectin mRNA in 3T3-L1 adipocyte expression was determined with semiquantity RT-PCR using beta-actin as internal reference. RESULT: The level of adiponetin mRNA in 3T3-L1 adipocyte was increased after treated with berberine only (P < 0.05), the effect of berberine was inhibited by high concentration insulin (P < 0.05). CONCLUSION: In vitro, berberine increases the expression of adiponectin in 3T3-L1 adipocyte, insulin inhibits the effect of berberine. |
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| Keywords: | berberine adiponectin insulin 3T3-L1 adipocyte |
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