| SD乳鼠离体骨骼肌成肌细胞与心肌细胞间连接的初步研究 |
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| 引用本文： | 赖颢,徐德民,虞勇,王春生,王克强. SD乳鼠离体骨骼肌成肌细胞与心肌细胞间连接的初步研究[J]. 中国临床医学, 2009, 16(2): 180-183 |
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| 作者姓名： | 赖颢 徐德民 虞勇 王春生 王克强 |
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| 作者单位： | 复旦大学附属中山医院心外科,上海,200032 |
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| 基金项目： | 上海市重点学科建设项目 |
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| 摘 要： | 目的：探寻共同培养的骨骼肌成肌细胞与心肌细胞间连接的形态学证据。方法：将鼠心肌细胞与骨骼肌成肌细胞体外共培养,于第2、4、7、14天免疫组化染色钙粘素（Cadherin）、细胞间隙连接蛋白73（Connexin43）、神经细胞粘附分子（NCAM）3种蛋白,并行定量RT-PCR检查分组比较分析;于第4、7、10、14天透射电镜观察。结果：共同培养物中的骨骼肌成肌细胞表达Connexin43,且随时间延长而增加;Cadherin表达增加不明显;NCAM含量维持在较低水平,至2周时才表达较多;透射电镜下观察到两者间存在类似于缝隙连接的连接。结论：与心肌细胞共培养后骨骼肌成肌细胞融合、成熟延迟,Connexin43表达增多;两者间确有形成类似于缝隙连接的物质基础并可形成连接。
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| 关 键 词： | 心肌细胞 骨骼肌成肌细胞 连接 |
Morphology Study of the Junction between Cocultured Skeletal Myoblasts and Cardiac Myocytes in vitro |
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| LAI Hao,XU Demin,YU Yong,WANG Chunsheng,WANG Keqiang. Morphology Study of the Junction between Cocultured Skeletal Myoblasts and Cardiac Myocytes in vitro[J]. Chinese Journal Of Clinical Medicine, 2009, 16(2): 180-183 |
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| Authors: | LAI Hao XU Demin YU Yong WANG Chunsheng WANG Keqiang |
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| Affiliation: | LAI Hao XU Demin YU Yong WANG Chunsheng WANG Keqiang Department of Cardiac Surgery,Zhongshan Hospital,Fudan University,Shanghai 200032 |
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| Abstract: | Objective:To investigate the morphological evidence for the connection of cocultured myoblasts and myocytes. Methods: Rat myocytes and skeletal myoblasts were cocultured with the ratio of 4:1. Cadherin,Connexin43 and NCAM were stained by ABC-AEC staining on day 2,4,7 and 14 respectively. The staining results were observed under microscope. Cells were compare d by transmission electroscope on day 4,7,10 and 14. Three groups were set as followed: experiment group (myocyte and myoblast were co-cultured with ra... |
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| Keywords: | Cardiac myocyte Skeletal myoblast Junction |
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