解毒通络注射液对缺血再灌注大鼠脑水肿的防治作用

目的：研究解毒通络注射液（Jiedu Tongluo injection，JDTL）对缺血性再灌注大鼠脑组织含水量变化以及细胞间黏附分子-1（ICAM-1）、血管细胞间黏附分子-1（VCAM-1）、E-选择素（E-selectin）、基质金属蛋白酶-9（MMP-9）表达的影响，探讨其对脑缺血再灌注损伤保护作用的机制。方法：线栓法建立大鼠大脑中动脉缺血再灌注模型，干-湿重法检测脑组织含水量；采用比色法测定脑组织中伊文思蓝（Evans blue，EB）含量反映血脑屏障通透性；酶联免疫吸附法（ELISA）检测脑组织中ICAM-1，VCAM-1，E-selectin表达；免疫组化法检测脑组织MMP-9表达。结果：JDTL 2，4 mL·kg^-1可明显降低模型大鼠的脑组织含水量，EB含量，ICAM-1，VCAM-1，E-selectin以及MMP-9的表达（P<0.05或P<0.01）。结论：解毒通络注射液对大鼠脑缺血再灌注损伤引起的脑水肿有保护作用，其作用机制与降低模型大鼠脑组织ICAM-1，VCAM-1，E-selectin的表达、抑制脑组织MMP-9的表达有关。

Protective effect of Jiedu Tongluo injection on cerebral edema in rats with lesion of cerebral ischemia/reperfusion

Objective: To investigate the protective effects of Jiedu Tongluo injection on cerebral edema induced by focal lesion of cerebral ischemia/reperfusion, the hydrous content of brain and the expressions of intercellular adhesion molecule-1(ICAM-1),vascular cell adhesion molecule-1 (VCAM-1),E-selectin and MMP-9 in rats. Method: The model of brain middle cerebral artery ischemia/reperfusion was established by the thread approach. After 24 hours of reperfusion, cerebral edema formation was determined by the hydrous content of brain. The permeability of blood brain barrier was evaluated based on the leakage of Evans blue. Enzyme-linked immunoadsordent assay (ELISA)was used to examine the expression of ICAM-1, VCAM-1, E-selectin. The expression of MMP-9 was measured by immunohistochemistry. Result: JDTL, in the dose of 2 mL·kg^-1 and 4 mL·kg^-1,relieved cerebral edema(P<0.05, P<0.01), reduced the expressions of ICAM-1, VCAM-1and E-selectin and decreased MMP-9 activity(P<0.05, P<0.01)in model rats. Conclusion: Jiedu Tongluo injection has a protective effect on rat brain from cerebral edema induced by the injury of focal cerebral ischemia/reperfusion. The mechanism is related to that Jiedu Tongluo injection can reduce the expressions of ICAM-1, VCAM-1 and E-selectin and inhibit of MMP-9 activation in rat brain.