紫外分光光度法测定雷公藤多苷片和不同产地药材中总生物碱的含量

目的：建立紫外分光光度法测定雷公藤多苷片中总生物碱含量的方法。测定不同厂家雷公藤制剂和不同产地雷公藤药材中总生物碱含量。方法：采用紫外分光光度法，以雷公藤吉碱为对照，在267nm波长处进行含量测定。结果：雷公藤吉碱在4．13～57．82μg·ml^-1范围内线性关系良好，r=0．9996。平均加样回收率为100．19％，RSD为2．19％（n=9）。不同厂家雷公藤制剂和不同产地雷公藤药材中总生物碱含量差异巨大。结论：该方法简便、快速、准确，适用于雷公藤制剂及药材中总生物碱的含量测定。

Determination of total alkaloids in leigongtengduogan pian and Tripterygium wilfordii from different regions by ultraviolet spectrophotometry

Objective： To establish a method for the determination of total alkaloids in leigongtengduogan pian by ultraviolet spectrophotometry, and determine the total alkaloids in leigongtengduogan pian and Tripterygium wilfordii from different regions. Methods： Using the wilforgine as the reference, the total alkaloids were determinated with the detection wavelength 267nm by UV. Results： For wilforgine in Tripterygium wilfordii, there were good linear relationship in the range of 4.13-57.82 p,g. ml^-1, r =0.999 6, with the average recovery 100.19% and RSD 2.19% （n =9）.The contents of alkaloids in Tripterygium wilfordii from different regions revealed a sharp difference. Conclusion： This method is simple ,quick and accurate. It can be used for the determination of total alkaloids in Tripterygium wilfordii of different preparation and also can be used as a quality control method of Tripterygium wilfordii.