胰岛素对新生大鼠缺氧/复氧心肌细胞凋亡的影响

目的:探讨胰岛素在新生大鼠缺氧/复氧心肌细胞损伤中对细胞凋亡的影响。方法:通过给原代培养乳鼠心室肌细胞行缺氧2 h/复氧4 h,建立缺氧/复氧(Anoxia/Reoxygenation)心肌细胞损伤模型。将培养心肌细胞随机分为:正常对照组(CON)、缺氧/复氧组(A/R)、缺氧/复氧+胰岛素组(I/R+INS)。于复氧4 h后,利用2,4二硝基苯肼显色法检测乳酸脱氢酶(LDH)活性,硫代巴比妥酸显色法检测丙二醛(MDA)含量,原位末端标记法(TINEL)和DNA梯带法(DNA Ladder)检测细胞凋亡,并比较各组间差异。结果:与A/R组相比,A/R+INS可明显降低MDA、LDH值(P<0.01),抑制心肌细胞凋亡(P<0.01)。结论:胰岛素具有减少新生大鼠缺氧/复氧心肌细胞损伤作用,其作用机制与抑制细胞凋亡作用有关。

Effect of Insulin Against Anoxia/Reoxygenation Induced Apoptosis in Cultured Cardiomyocyte of Neonatal Rat

Objective: To study protective effect of insulin against Cardiomyocyte apoptosis in anoxia/reoxygenation(A/R) injury of neonatal rat. Method: The model of A/R injury was finished through receiving anoxia for 2 hours and re-oxygenation for 4 hours in cultured cardiomyocytes of neonatal rat. The car-diomyocytes were randomized to divided into 3 groups: control group (CON), anoxia/reoxygenation group (A/R) and anoxia/reoxygenation insulin group(A/R INS). At the end of reoxygenation of 4 hours, activities of lactate dehydrogenase (LDH), content of malondialdehyde (MDA) were assayed through spectrophotometric procedures,myocyte apoptosis were detected through TUNEL and DNA Ladder. Results: MDA, LDH and Apoptosis index is significantly decreased in A/R INS group compared with A/R group( P < 0.01). Conclusion: Insulin has a protective effect against A/R injury in cultured Cardiomyocyte of neonatal rat,the protective mechanism may contribute to antiapoptosis of insulin.