| 不同产地党参HPLC指纹图谱研究及比较 |
| |
| 引用本文： | 刘晓芳,赵慧辉,刘养清,程若敏,刘小玉,王秀文,焦建华.不同产地党参HPLC指纹图谱研究及比较[J].山西中医学院学报,2010,11(1):28-29,60. |
| |
| 作者姓名： | 刘晓芳 赵慧辉 刘养清 程若敏 刘小玉 王秀文 焦建华 |
| |
| 作者单位： | 1. 山西中医学院,山西,太原,030024
2. 北京中医药大学,北京,100029 |
| |
| 基金项目： | 山西省卫生厅科技攻关项目 |
| |
| 摘 要： | 目的:研究不同产地党参的指纹图谱。方法:采用反相高效液相色谱法,色谱柱:Grace Smart RP C18柱(250nm×4.6nm,5μm),以乙腈-0.1%醋酸为流动相进行二元梯度洗脱,流速0.6mL/min,检测波长268nm,柱温30℃。结果:党参样品HPLC指纹图谱可明确显示不同党参样品的差异性和相似性,精密度、稳定性和重复性实验的RSD均小于3%,用党参炔苷作为参照物可确认党参样品中的活性成分。结论:本方法操作简便,结果稳定可靠,且利用指纹图谱的整体性,可以对不同产地党参药材进行真伪鉴别,并对质量进行定性、定量评价。
|
| 关 键 词： | 党参 党参炔苷 指纹图谱 高效液相色谱 |
Study and Comparison on HPLC Finger Printing of Radix Codonopsitis from Different Habitats |
| |
| Liu Xiaofang,Zhao Huihui,Liu Yangqing,Cheng Ruomin,Liu Xiaoyu,Wang Xiuwen,Jiao Jianhua.Study and Comparison on HPLC Finger Printing of Radix Codonopsitis from Different Habitats[J].Journal of Shanxi College of Traditional Chinese Medicine,2010,11(1):28-29,60. |
| |
| Authors: | Liu Xiaofang Zhao Huihui Liu Yangqing Cheng Ruomin Liu Xiaoyu Wang Xiuwen Jiao Jianhua |
| |
| Institution: | Liu Xiaofang1,Zhao Huihui2,Liu Yangqing1,Cheng Ruomin1,Liu Xiaoyu1,Wang Xiuwen1,Jiao Jianhua1(1.Shanxi College of TCM,Taiyuan Shanxi 030024,2.Beijing University of TCM,Beijing 100029) |
| |
| Abstract: | Objective：To establish finger printing of Radix Codonopsitis from different habitats. Method： HPLC was performed on Grace Smart-C18 column（4.6 nm×250 nm,5 μm）with acetonitrile-0.1% acetic acid as mobile phase. The detection wavelength was set 268 nm, flow rate was at 0. 6 mL/min, column temperature was at 30 ℃. Result：Finger printing could demonstrate obviously difference and similarity of Radix Codonopsitis from different habitats. The RSD of precision trial, stability trial and reproducibility trial was lower than 3%. Conclusion ：The method was simple ,reproducible and reliable, and can be used to identify the authenticity of Radix Codonopsitis from different habitats. |
| |
| Keywords: | Radix Codonopsitis finger printing HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
