荜茇宁对家兔实验性动脉粥样硬化的影响

目的:观察荜茇宁对家兔实验性动脉粥样硬化(AS)形成的影响,并探讨其作用机制。方法:采用高脂饲料喂养建立家兔AS模型,将兔随机分为5组:正常组,模型组,荜茇宁高、低剂量组分别给予荜茇宁5,2.5 mg.kg^-1.d^-1,辛伐他汀组给予辛伐他汀5 mg.kg^-1.d-^-1,连续喂养60 d。于实验前1 d及开始后第20,40,60天取空腹血检测血清总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C),第60天检测血清超氧化物歧化酶(SOD)活力和丙二醛(MDA)、一氧化氮(NO)含量,随后处死动物,取主动脉和心脏做病理形态学检查。结果:与模型组相比,荜茇宁组的血清TC,TG,LDL-C水平降低(P<0.05或0.01),HDL-C有升高趋势,SOD,NO升高(P<0.05),MDA降低(P<0.05)。荜茇宁组主动脉斑块面积占总面积的百分比显著低于模型组(P<0.01);主动脉、心脏损伤程度减轻,冠状动脉狭窄程度轻于模型组(P<0.01);透射电镜观察显示荜茇宁组主动脉的超微结构病变程度明显轻于模型组。结论:荜茇宁能抑制AS斑块的形成和发展,其作用机制可能与调节血脂、抗氧化有关。

Antiatherogenic effect of piperlonguminine on experimental atherosclerosis in rabbits

Objective: To investigate of antiatherogenic effect and possible mechanisms of piper longuminine.Method: The atherosclerotic model was established by the hypercholesterol feeding rabbits.Male Mew Zealand rabbits were randomly divided into five groups: normal group,model group,the high-dose(5 mg·kg^-1·d^-1) and low-dose(2.5 mg·kg^-1·d^-1) group of piperlonguminine,and simvastatin group(5 mg·kg-1·d-1).All the rabbits were fed for 60 days.Blood samples were taken from the ear edge vein of rabbits in the day before the experiment,and in the days of 20,40 and 60 days after the experiment,respectively.All the rabbits were fasted for at least twelve hours before the blood was taken. The blood serum were analyzed for total cholesterol(TC),triglyceride(TG),low density lipoprotein cholesterol(LDL-C) and high-density lipoprotein cholesterol(HDL-C).The blood serum of the 60th day were also analyzed for superoxide dismutase(SOD),malondialdehyde(MDA) and nitric oxide(NO).At last,the pathological observation of aorta and heart samples were carried out.Result: Compared with those in model group,the TC,TG and LDL-C levels were reduced(P<0.05) and the HDL-C was raised in the piperlonguminine group;also,the serum SOD and NO level was raised(P<0.05),MDA level was reduced in the piperlonguminine group(P<0.05).Area percentage of aorta plaque was reduced(P<0.01) in the piperlonguminine group.The aorta and heart injury was abated and coronary artery angusty extent was markedly abatement(P<0.01).The results of observation through transmission electron microscope(TEM) indicated that the fine structure of aortal pathological degree was markedly abated.Conclusion: The piperlonguminine could inhibit the atherogenesis formation and development,which might be due to regulating the lipid metabolism and enhancing the antioxidation.