| 肝细胞生长因子对长时间保存大鼠供体心bcl-2mRNA表达及细胞凋亡的影响 |
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| 引用本文： | 吴巧艺,林峰,陈道中.肝细胞生长因子对长时间保存大鼠供体心bcl-2mRNA表达及细胞凋亡的影响[J].中国医药,2008,4(1):489-490. |
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| 作者姓名： | 吴巧艺 林峰 陈道中 |
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| 作者单位： | 福建医科大学附属第一医院急诊外科,福州,350005;福建医科大学附属协和医院心外科,福州,350005; |
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| 摘 要： | Objective To observe the effect of hepatocyte growth factor on isolated rat heart preservation. Methods Forty SD mrs were randomly divided into two groups : control group (n = 20) with isolated rat heart preserved in 4 ℃ HTK solution and experimental group(n =20) with isolated rat hearts preserved in 4 ℃ HTK + rh-HGF(100 μg/L) solution. Cell apoptosis was detected by TUNEL staining and the expression of bcl-2mRNA was examined by RT-PCR. Results Experimental group showed a lower rate of apoptosis eardiomyocytes (P < 0.01) after reperfusion. Immunohistochemieal analysis revealed that c-met receptor expression was stronger in the HGF-treated myocardium than that in the non-HGF-treated myocardium after storage and was associated with a stronger ex-pression of bcl-2 mRNA. Conclusion The administration of rh-HGF before storage improved cardiac function after prolonged myocardial preservation by preventing apoptosis and enhancing expression of bcl-2 mRNA.
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| 关 键 词： | 心脏移植 肝细胞生长因子 心脏保存 |
Effect of recombinant human hepatocyte growth factor on bcl-2 mRNA expression and cell apoptosis during long-time heart graft preservation in rats |
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| Abstract: | |
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| Keywords: | Heart transplantationHepatocyte growth factorHeart preservation |
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