转染骨形态发生蛋白-2基因的人骨髓间质干细胞复合异种骨支架异位成骨的效果

目的 观察骨形态发生蛋白-2(BMP-2)罐因转染的人骨髓间质干细胞复合异种骨支架异位成骨效果。方法 分组：(1)BMP-2基因转染细胞 异种骨支架[去抗原牛松质骨(BCB)]；(2)对照基因转染细胞 BCB；(3)未转染细胞 重组BMP-2 BCB；(4)未转染细胞 BCB；(5)BCB。分别将各组人工骨植入裸鼠皮下，于术后4，8周行组织学观察．结果 体内植入后3d，细胞持续表达外源基因BMP-2基因转染组8周时完全由新形成的编织骨构成，血管丰富。结论 BMP-2基因转染后细胞复合BCB支架，可以在异位形成骨组织并诱导毛细血管长入。

Heterotopic osteogenesis induced by xenogeneic bone delivery of human mesenchymal stem cells transfected by bone morphogenetic protein-2 gene in nude mice

Objective To evaluate the heterotopic osteogenenic potential of xenogeneic bone delivery of human mesenchymal stem cells transfected with bone morphogenetic protein-2 (BMP-2) gene. Methods Cell-scaffold complexes were implanted subcutaneously into the back of the nude mice in five groups respectively,ie,Ad-BMP-2 infected hBMSC plus antigen-free bovine cancellous bone (BCB) (Group A),Ad-LacZ infected hBMSC-BCB (Group B),hBMSC-BCB plus reconstructed hBMP-2 (Group C),hBMSC-BCB (Group D) and only BCB scaffolds (Group E). Bone formation and vascularization were observed by histological examination four and eight weeks after operation. Results hBMSC continuously expressed exogenous gene in vivo three days after implantation. The Group A was filled with newly formed woven bone with abundant blood capillary eight weeks after operation. Conclusions Xenogeneic bone delivery of human mesenchymal stem cells transfected by BMP-2 gene can attain heterotopic osteogenesis and induce vascularization of capillary vessels.