Method for studying immunologic cell-mediated response to transplanted syngeneic, allogeneic and xenogeneic fibroblasts in mice

A method is described for studying the types of host cells which react with transplanted xenogeneic, allogeneic and syngenetic fibroblasts. Fibroblasts were placed in an 0.15 mm glass fissure, thus limiting the wound reaction and making possible study of host cells infiltrating into the chamber. Under the conditions of the method, in early stages of the reaction to xenogeneic and allogeneic fibroblasts, mostly granulocytes migrated into the transplanted fissures, whereas syngeneic fibroblasts caused infiltration of only a small number of granulocytes.