复方浙贝药物血清影响K562/A02细胞积聚外排功能和细胞凋亡研究

目的 观察复方浙贝药物血清对K562/A02细胞积聚外排功能和细胞凋亡的影响。方法 Wistar 雄性大鼠40只, 分为空白对照组（血清组）、含阿霉素（ADR）血清组及含复方浙贝颗粒高［8 g/（kg·d）］、中［4 g/（kg·d）］、低［2 g/（kg·d）］剂量血清组。用流式细胞仪定量检测不同时间药物血清干预后的K562/A02细胞内ADR和罗丹明123（Rh123）浓度；Annexin V/PI双染法检测K562/A02细胞早期凋亡率。结果 复方浙贝药物血清不同剂量组均能减缓K562/A02细胞内ADR和Rh123荧光下降速度，其中高剂量组作用最明显，中剂量组次之，低剂量组较弱；K562/A02细胞早期凋亡率依次为：中剂量组24.60％、高剂量组11.21％、低剂量组5.71％、空白组1.40％。结论 复方浙贝颗粒体外能够抑制K562/A02细胞对药物的外排功能，并能够诱导细胞凋亡。

Study on the Compound Fritillaria thunbergii Miq Particles to the Subcellular Accumulation Ability and Inducing Apoptosis of K562/A02 Cells

Objective To investigate the effects of the Compound Fritillaria thunbergii Miq Particles(CFMP) to the sub-cellular accumulation ability and inducing the apoptosis of K562/A02 cells,explore the mechanism of the CFMP reserve the multi-drug resistance of K562/A02 cells. Methods Forty Wistar male rats were divided into the blank-serum group,the adriamycin (ADR) containing serum group,the CFMP high dose [8 g/(kg·d)] group,the CFMP medium dose [4 g/(kg·d)] and the CFMP low dose [2 g/(kg·d)] group. The effects of different time of drug contained serum on sub-cellular accumulation of ADR and Rh123 were quantitative detected by flow cytometry. Cells apoptosis induced by different concentrations of CFMP contained serum were detected by flow cytometry with Annexin-V/PI staining method. Results Different concentrations of CFMP contained serum can inhibit the descend trend of the ADR and Rh123 fluorescence accumulation curve of K562/A02 cells with dose-dependence. The high-dose group showed superior inhibition than the other. Annexin-V/PI staining method exhibited that the rate of apoptosis of K562/A02 cells in blank-serum group was 1.40%. Different concentrations of CFMP contained serum induced the apoptosis of K562/A02 cells showed a dose-dependent manner,that is medium dose group presented 24.60% apoptosis,high dose group presented 11.21% apoptosis,and low dose group presented 5.71% apoptosis. Conclusion CFMP can inhibit the descend trend of the ADR and Rh123 fluorescence accumulation curve of K562/A02 cells,induce the apoptosis of K562/A02 cells to achieve the reverse intention of multi-drug resistance.