Activity of ectonucleotidases and adenosine deaminase in rats exposed to cigarette smoke |
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| Authors: | G.R. Thomé C.M. Mazzanti M. Ahmed M. Corrêa R.M. Spanevello P.A. Maldonado |
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| Affiliation: | 1. Programa de Pós Gradua??o em Bioquímica Toxicológica, Centro de Ciências Naturais e Exatas, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil;2. Programa de Pós Gradua??o em Bioquímica, Centro de Ciências Biológicas Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil |
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| Abstract: | Cigarette smoke is a complex mixture of various toxic substances that are capable of initiating oxidative damage and promoting blood platelet alterations. In this study, we investigated the activities of the ectoenzymes NTPDase (ectonucleoside triphosphate diphosphohydrolase, CD39) and 5′-nucleotidase (CD73) in platelets as well as adenosine deaminase (ADA) in the plasma of rats exposed to aged and diluted sidestream smoke during 4 weeks. The rats were divided into two groups: I (control) and II (exposed to smoke). After the exposure period, blood was collected and the platelets and plasma were separated for enzymatic assay. The results demonstrated that NTPDase (with ATP as substrate) and 5′-nucleotidase (AMP as substrate) activities were significantly higher in group II (p?p?p?
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| Keywords: | Platelets NTPDase 5′-nucleotidase adenosine deaminase cigarette smoke rat |
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