大鼠视网膜节细胞细胞色素氧化酶与逆行示踪辣根过氧化物酶联合反应

大鼠经双上丘辣根过氧化物酶埋藏后,用氯化钴细胞色素氧化酶反应法处理视网膜,观察到节细胞分别呈高细胞色素氧化酶活性反应、辣根过氧化物酶标记和细胞色素氧化酶/辣根过氧化物酶联合反应,说明此法可作联合反应法使用。本实验中辣根过氧化物酶标记是在不使用过氧化氢的条件下作出的。

COMBINED HRP TRACING AND CYTOCHROME OXIDASE TECHNIQUE IN RETINAL GANGLION CELL

After the HRP had been embedded into the bilateral superior colliculi of the rats, a double reaction of the cytochrome oxidase and the retrograde HRP tracing of the retinal ganglion cells were successfully studied by means of cobalt chloride cytochrome oxidase technic. The results showed that the pure high cytochrome oxidase activity the HRP-labeled and the double reaction ganglion cells were revealed even in the same microscopic field. As a pure high cytochrome oxidase activity ganglion cell, the blue-grey colour product of the enzyme appeared within its perikaryon and processes. No visible cytochrome oxidase product, except the brown HRP grains could be found in the perikaryon and processes of the HRP labeled ganglion cells. The double reaction ganglion cells were demonstrated by the HRP grains emerged the from background of cytochrome oxidase product within its perikaryon and processes. Hydrogen peroxide was cancelled in this techenic.