大豆异黄酮对代谢综合征大鼠主动脉内皮细胞功能影响的实验研究

目的：观察大豆异黄酮对膳食诱导代谢综合征大鼠血管内皮功能的影响。方法：采用高脂高糖高盐饲料喂养建立大鼠MS模型。测量体重、血压、空腹血糖、血清胰岛素、血脂、HOMA-IR,筛选MS成模大鼠,予大豆异黄酮高、低剂量灌胃4周,以二甲双胍作为阳性药对照,检测主动脉H染色、血清vWF和eNOSmRNA表达情况。结果：模型组大鼠主动脉组织病理改变明显,SI高剂量组和二甲双胍组主动脉病理改变有不同程度改善。SI高剂量组和二甲双胍组体重、血糖、血清胰岛素和胰岛素抵抗指数较模型组均下降（P〈0.05）;仅SI高剂量组血压下降（P〈0.05）;SI高剂量组大鼠TG、TC和LDL-C水平均显著下降（P〈0.05）;三组大鼠血清vWF水平均降低（P〈0.05）,SI高剂量优于低剂量。在eNOS mRNA表达上SI高剂量组和二甲双胍组均较模型组增高（P〈0.05）。结论：大豆异黄酮能改善MS大鼠主动脉组织结构,降低血清vWF水平,上调主动脉eNOS mRNA表达发挥主动脉内皮保护作用。

The Influence Reseach of Soy Isoflavones on Endothelial Function of Metabolic Syndrome Rats

Objective： to observe the effection of soy isoflavones on vascular endothelial function in diet-induced metabolic syndrome rats.Methods： We established MS model by feeding rats high-fat-high-sugar-high-salt food.Measure body weight,blood pressure,fasting blood glucose,serum insulin,lipids,HOMA-IR choose MS model rats and gavage them high and low-dose soy isoflavones for 4 weeks.detect aortic H staining,expression of vWF and eNOSmRNA.Metformin was a positive control drug.Results： The pathological change was obvious in model group rats aorta,and that in SI high-dose group and metformin group had improvement in varying degree.Body weight,blood glucose,serum insulin and insulin resistance index in SI high-dose group and metformin group model decreased obviously compared with model group（P〈0.05）;Blood pressure decreasing noticeably only happened in SI high-dose group（P〈0.05）;TG,TC and LDL-C were significantly decreased in SI high-dose group rats（P〈0.05）;The serum vWF level in those three groups was lower（P〈0.05）,but SI high-dose group was superior to the low-dose one.On the expression of eNOS mRNA in SI high-dose group and metformin group increased than that in model group（P〈0.05）.Conclusion： Soy isoflavones can improve the aortic structure in MS rat,reduce the serum vWF levels,increase eNOS mRNA expression and protect aortic endothelial function.