钻原卟啉诱导血红素氧合酶-1表达对豚鼠银屑病样模型的影响

目的：确定钴原卟啉（CoPP）诱导血红素氧合酶-1（heme oxygenase-1,HO-1）对豚鼠银屑病样皮损的影响.方法：普萘洛尔外涂构建豚鼠的银屑病样模型,豚鼠腹腔内注射钴原卟啉（HO-1特异性诱导剂）或锌原卟啉（HO-1特异性抑制剂）,磷酸盐缓冲液（phosphate buffered saline, PBS）作为对照, 每周1次,共6周.记录皮损的动态评分和组织病理学评分.RT-PCR和Western-blot法检测动物耳部标本中的HO-1 mRNA和蛋白的表达.免疫组化染色方法检测标本中增殖细胞核抗原（proliferating cell nuclear antigen, PCNA）蛋白的表达和定位.结果：CoPP腹腔内注射显著诱导了HO-1 mRNA和蛋白的表达, CoPP治疗组银屑病样皮损的组织病理学评分和临床皮肤评分显著降低,与PBS对照组相比有显著性差异（P〈0.05）,同时CoPP治疗组的PCNA表达也显著低于PBS对照组（P〈0.05）.结论：钴原卟啉通过诱导血红素氧合酶-1的过表达对银屑病样皮损有治疗作用.

Effect of heme- oxygenase- 1 induced by cobalt proporphyrin on psoriasis model in guinea pigs

Objective： To determine the effect of heme - oxygenase - 1 （HO - 1 ） induced by cobalt proporphyrin （CoPP） on psoriasislike skin lesions. Methods： After a psoriasiform model of guinea pig was established using topical propranolol, cobalt proporphyrin （a HO- 1 inducer） or zinc proporphyrin （ZnPP）, a HO- 1 inhibitor was intraperitoneally injected, once a week for six weeks. Phosphate buffered saline （PBS） was used as control. The clinical skin score and the histopathological score were noted. The level of HO - 1 mRNA and protein was assessed by RT - PCR and Western - blot. The expression of proliferating cell nuclear antigen （PCNA） protein was measured by immunohistochemical staining. Results： The expression of HO - 1 mRNA and protein was significantly increased, and the clinical and pathological scores of psoriasiform skin lesions was significantly decreased in CoPP group, when compared with PBS group （ Ps 〈 0.05）. The expression of PCNA in the CoPP group was significantly less than that in PBS group. Conclusion： PPCoPP can resolve psoriasiform skin lesions by inducing HO- 1 over- expression.