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大花红景天药材的HPLC指纹图谱研究
引用本文:郭晓蕾,翟旭峰,王怀豫,宝丽.大花红景天药材的HPLC指纹图谱研究[J].中医药学刊,2010(6):1317-1319.
作者姓名:郭晓蕾  翟旭峰  王怀豫  宝丽
作者单位:[1]无限极(中国)有限公司,广东广州510665 [2]中国科学院微生物研究所真菌地衣系统学实验室,北京100101
摘    要:目的:利用高效液相色谱法建立了大花红景天的色谱指纹图谱。方法:固定相采用C18反相色谱柱,流动相为甲醇-0.04%三氟乙酸水溶液梯度洗脱,检测波长280nm,流速为1.0mL/min。结果:通过比较发现大花红景天样品的6个共有峰,可作为鉴定大花红景天药材的主要依据。结论:方法简便快速,为大花红景天药材的鉴定和质量控制提供了较全面的信息。

关 键 词:高效液相色谱  大花红景天  指纹图谱

HPLC Chromatographic Fingerprints of Rhodiola Crenulata
GUO Xiao-lei,ZHAI XU-feng,WANG Huai-yu,BAO Li.HPLC Chromatographic Fingerprints of Rhodiola Crenulata[J].Study Journal of Traditional Chinese Medicine,2010(6):1317-1319.
Authors:GUO Xiao-lei  ZHAI XU-feng  WANG Huai-yu  BAO Li
Institution:1.Infinitus(China) Company Ltd,Guangzhou 510665,Guangdong,China;2.Key Lab of Systematic Mycology and Lichenology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China)
Abstract:Objective:The chromatographic fingerprint of Rhodiola crenulata was established by high performance liquid chromatography (HPLC). Methods:The separation method involves a RP-C18 column,a mobile phase of 0.04% TCA:CH3OH,an ultraviolet detector at 280 nm and a flow speed of 1.00 mL/min. Results:According to the comparative study of chromatographic fingerprints,we found 6 communal peaks,which can be applied to identify Rhodiola crenulata. Conclusion:This method is helpful to control the quality of this herb.
Keywords:HPLC  Rhodiola crenulata  fingerprint
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