凋亡相关基因在非小细胞肺癌组织中表达的研究

目的　探讨ｂｃｌ２ 、ｂａｘ、Ｆａｓ、ＦａｓＬ凋亡相关基因在非小细胞肺癌中表达及与肿瘤临床病理学的关系。方法　采用免疫组织化学方法检测４５ 例非小细胞肺癌中ｂｃｌ２、ｂａｘ、Ｆａｓ 和ＦａｓＬ的表达。结果　２３ 例（５１ ％）ｂｃｌ２ 阳性，３８ 例（８４％ ）ｂａｘ 阳性（ Ｐ＜ ０－０１） ，二者之间表达无明显关系，但Ｆａｓ 与ＦａｓＬ之间表达呈正相关（Ｐ＜０－０１） 。ｂｃｌ２ 蛋白表达率随肿瘤期别进展而降低（ Ｐ＜ ０－０５） ，ｂａｘ 蛋白在低分化癌中表达减少（Ｐ＜０－０５） ，ＦａｓＬ在肺腺癌细胞中表达较高（Ｐ＜ ０－０１） 。ｂｃｌ２ 和ｂａｘ 蛋白同时表达阳性与肺癌ＴＮＭ 分期显著相关（Ｐ＜０－０５） ，ｂｃｌ２ 蛋白单一表达与细胞分化有关（ Ｐ＝０－０３３）。结论ｂｃｌ２、ｂａｘ、Ｆａｓ 和ＦａｓＬ可能通过不同途径参与了非小细胞肺癌组织中细胞凋亡的调节，并在肿瘤的发生与发展中发挥重要作用。

Study on expression of apoptosis related genes in non small cell lung carcinoma

Objective To study the relationship between the expression of apoptosis related genes in the tissue of non small cell lung carcinoma (NSCLC) and clinicopathology in the tumor Methods The expression of bcl 2, bax, Fas and FasL were detected with immunohistochemistry method in 45 cases of NSCLC Results 23(51%) cases of NSCLC were bcl 2 positive, 38 (84%) were bax positive ( P <0 01), and there was no significant correlation between the expression of bcl 2 and bax protein, but a positive relationship was found between the expression of Fas and FasL ( P <0 01) The positive rate of bcl 2 expression decreased in advanced stages of NSCLC( P <0 05),the bax expression was lower in cases with poor cell differentiation ( P <0 05),and the FasL expression was higher in adenocarcinoma ( P <0 01) The simultaneous expression of bcl 2 and bax protein was closely correlated with TNM stages ( P <0 05), the differences of bcl 2 protein expression alone in NSCLC with cell differentiation were statistically significant ( P =0 033) Conclusions Both bcl 2, bax, Fas and FasL may involve in the regulation of apoptosis and may play an important role in the occurrence and progression of NSCLC