PTD-BCR/ABL SH3融合蛋白对肝癌细胞系HHCC的体外杀伤活性的研究

目的 探讨PTD—BCR／ABL SH3融合蛋白对肝癌细胞系HHCC的体外杀伤活性。方法 通过将PTD—BCR／ABL SH3融合蛋白与HHCC细胞共培养。用免疫组织化学染色的方法观察和检测PTD—BCR／ABL SH3融合蛋白进入细胞后的定位，MTT法检测蛋白对肝癌细胞的杀伤活性，电镜观察HHCC肝癌细与PTD—BCR／ABL SH3融合蛋白共培养后超微结构变化。结果 PTD—BCR／ABL SH3融合蛋白与肿瘤细胞共培养后，免疫组织化学染色表明蛋白进入肝癌细胞后主要分布在细胞核．胞浆少量分布。MTT检测发现与同期空白对照相比，实验组细胞数量显著减少，提示蛋白可抑制细胞生长。电镜观察显示肝癌细胞在PTD—BCR／ABL SH3融合蛋白的作用下呈现明显的凋亡细胞特征。结论 PTD—BCR／ABL SH3融合蛋白可以引发肝癌细胞凋亡从杀伤肿瘤细胞，为将该蛋白运用于肝肿瘤的治疗奠定了基础。

IN VITRO STUDY ON KILLING ACTIVITY OF PTD-BCR/ABL SH3 FUSION PROTEIN TO HEPATOCELLULAR CANCER CELL LINE HCC

Objective To explore the cytotoxicity of PTD BCR/ABL SH3 fusion protein to hepatocellular cancer cell line HHCC in vitro.Methods HHCC cells were cocultured with PTD BCR/ABL SH3 fusion protein.The localization of PTD BCR/ABL SH3 fusion protein was observed and detected by immuno histochemical staining transmission.The cytotoxicity was measured with method of MTT.Electron microscope was used to observe the changes of ultrastructure of HCC cells after coculturing with PTD BCR/ABL SH3 fusion protein.Result After HCC cells cocultured with PTD BCR/ABL SH3 fusion protein.Immuno histochemical staining presented localization of the protein was mainly in nucleus,even some in cytoplasm.Compared with antitheses,the cell's number of experimented groups declined obviously,which clued that the protein could inhibit proliferation of the cells.Electronic micro photos showed that PTD BCR/ABL SH3 fusion protein induced HHCC cells to obvious characters of apoptosis cells.Conclusion PTD BCR/ABL SH3 fusion protein has obviously killing activity of inducing HHCC cells unspecific apoptosis in vitro.It is a good basis established for using the protein to cure hepatocellular cancer.