外周血HBV cccDNA检测的临床应用

目的通过对乙型肝炎患者外周血单个核细胞及血浆中共价闭合环状DNA（cccDNA）的检测，初步探讨其外周血检测对乙型肝炎诊治的价值。方法将86例确诊为乙型肝炎患者根据病情分为3组，急性乙型肝炎（AHB1、慢性乙型肝炎（CHB）及HBsAg无症状携带者（ASC）组。采集外周血，分别检测ALT、AST、总胆红素（TBIL）、cccDNA、HBV—DNA。结果三组血浆cccDNA检测结果有显著性差异（P〈0．01）。单个核细胞cccDNA7个标本阳性，血浆cccDNA与HBV—DNA、ALT、AST、TBIL相关系数分别为0．817、0．402、0．450、0．221。结论血浆cccDNA在乙型肝炎患者的各个病程有显著性差异，单个核细胞cccDNA储存池确实存在但浓度较低难以检测。血浆cccDNA．与HBV—DNA、ALT、AST相关，但与TBIL无明显相关。

Clinical apply of peripheral blood HBV cccDNA detection

Objective To detect both peripheral blood mononuclear and plasma covalently closed circular DNA(cccDNA) of HBV patients,to investigate peripheral blood cccDNA value for HBV diagnosis and treatment.Methods Divided 86 cases of HBV patients into three groups,acute hepatitis B(AHB),chronic hepatitis B(CHB),HBsAg asymptomatic carriers(ASC) group.Peripheral blood was collected,we detected ALT,AST,TBIL,cccDNA and HBV-DNA,then analyzed the result with SPSS 11.0.Results With rank sum test,there were significant difference for cccDNA among three groups(P<0.01).Seven samples was positive for peripheral blood mononuclear,plasma HBV-DNA and cccDNA,ALT,AST,TBIL correlation coefficients were 0.817,0.402,0.450,0.221.The cccDNA in the plasma of all patients with hepatitis B disease were significantly different,mononuclear cells cccDNA storage pool does exist but it’s low and difficult to detect.Conclusion Concentration of plasma cccDNA is related with HBV-DNA,ALT,AST,but no significant correlation with TBIL.