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| CBN对脑缺血再灌注小鼠线粒体损伤的影响 | |
| 引用本文： | 金文,何希辉,潘卫松,孙虹,杜力军. CBN对脑缺血再灌注小鼠线粒体损伤的影响[J]. 中药药理与临床, 2003, 19(4): 32-34 |
| 作者姓名： | 金文 何希辉 潘卫松 孙虹 杜力军 |
| 作者单位： | 1. 中国医学科学院,中国协和医科大学,药用植物研究所,北京,100094 2. 清华大学,生物科学与技术系,北京,100084 |
| 基金项目： | 国家新药研究基金资助 ,合同号 96 90 1 0 5 1 0 5 |
| 摘 要： | 目的：观察CBN对脑缺血再灌注小鼠的线粒体损伤的影响。方法：分离提纯脑缺血再灌注小鼠脑的线粒体。用分光光度法检测线粒体的膜肿胀度，用激光拉曼光谱检测脑线粒体的生物大分子的变化。结果：小鼠脑缺血再灌注后。其线粒体出现膜肿胀，CBN50、100mg／kg iv对此有明显的抑制作用。各组脑线粒体的Raman和IR光谱基本一致。CBN组与假手术组比较接近。模型组只是在量上有一些差异。结论：CBN对脑缺血再灌注小鼠的线粒体损伤的有一定的保护作用。 |
| 关 键 词： | 脑缺血再灌注 线粒体损伤 CBN 金森脑泰粉针剂 中药制剂 |
Protection of CBN Injection from the mouse brain''''s mitochrodria damaged by ischemia-reperfusion |
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| Jin Wen,He Xihui,Pan Weisong,Sun Hong,Du Lijun. Protection of CBN Injection from the mouse brain''''s mitochrodria damaged by ischemia-reperfusion[J]. Pharmacology and Clinics of Chinese Materia Medica, 2003, 19(4): 32-34 | |
| Authors: | Jin Wen He Xihui Pan Weisong Sun Hong Du Lijun |
| Affiliation: | Jin Wen,He Xihui,Pan Weisong,Sun Hong,Du Lijun 1 |
| Abstract: | Objective: To study the effect of CBN from the mouse brain's mitochrodria damaged by ischemia-reperfusion. Methods: The mitochondria of cerebral ischemia-reperfusion brain was isolated, the mitochondria membrane swelling are determined by spectrometry, the change of molecular are determined by Raman and IR spectrum.. Results: The mitochondria of cerebral ischemia-reperfusion brain showed that the mitochondria membrane swelling significantly, and CBN had protective effect on mitochondria membrane which play a important role in mitochondria barrier function. From the Raman and FT-IR spectroscope of mitochondria, we obversed some changes in the molecular conformation of mitochondria of cerebral ischemia reperfusion brain, and CBN could alleviate them. Conclusion: It is suggested that CBN can protect the mouse brain's mitochrodria damaged by ischemia-reperfusion. |
| Keywords: | CBN ischemia-reperfusion mitochondria membrane swelling Raman spectrum IR spectrum |
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