Protection of PC12 Cells against Superoxide-induced Damage by Isoflavonoids from Astragalus mongholicus |
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Authors: | DE-HONG YU YONG-MING BAO LI-JIA AN MING YANG |
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Institution: | [1]The State Key Laboratory of Natural and Biomimetic Drugs, Peking University, Beijing 100083 China; [2]Department of Bioscience and Biotechnology, Dalian University of Technology, Dalian 116024, Liaoning, China |
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Abstract: | Objective To further investigate the neuroprotective effects of five isoflavonoids from Astragalus mongholicus on xanthine (XA)/xanthine oxidase (XO)-induced injury to PC12 cells. Methods PC12 cells were damaged by XA/XO. The activities of antioxidant enzymes, MTT, LDH, and GSH assays were used to evaluate the protection of these five isoflavonoids. Contentsof Bcl-2 family proteins were determined with flow cytometry. Results Among the five isoflavonoids including formononetin, ononin, 9, 10-dimethoxypterocarpan-3-O-β-D-glucoside, calycosin and calycosin-7-O-glucoside, calycosin and calycosin-7-O-glucoside were found to inhibit XA/XO-induced injury to PCI2 cells. Their EC50 values of formononetin and calycosin were 0.05 μg/mL. Moreover, treatment with these three isoflavonoids prevented a decrease in the activities of antioxidant enzymes, superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), while formononetin and calycosin could prevent a significant deletion of GSH. In addition, only calycosin and calycosin-7-O-glucoside were shown to inhibit XO activity in cell-free system, with an approximate IC50 value of 10 μg/mL and 50 μg/mL. Formononetin and calycosin had no significant influence on Bcl-2 or Bax protein contents. Conclusion Neuroprotection of formononetin, calycosin and calycosin-7-O-glucoside may be mediated by increasing endogenous antioxidants, rather by inhibiting XO activities or by scavenging free radicals. |
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Keywords: | Astragalus mongholicus Neuroprotection Xanthine/xanthine oxidase PC12 cells |
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