湖北海棠总黄酮纯化工艺研究

目的 优化湖北海棠总黄酮分离纯化工艺.方法 以总黄酮含量为评价指标,采用大孔吸附树脂法进行纯化,确定总黄酮纯化工艺条件.结果 湖北海棠总黄酮最佳分离纯化工艺为：选用HPD100型大孔树脂,树脂与药材的质量比为1∶1.74,上样药液浓度为0.15g/ml,上样流速为2BV/h,树脂径高比为1∶10,洗脱时先用4BV水洗除杂,再用4BV70％乙醇洗脱,洗脱速度为1BV/h.结论 纯化工艺简便、可行,适合工业化生产.

Study on Purification for Total Flavone of Malus Hupehensis

Objective To optimize separation and purification for total flavone of Malus hupehensis.Methods Taking the total flavone content as evaluation index,we determined purification condition of total flavone by selecting macroporous absorption resin.Results The optimum separation and purification for total flavone of Malus hupehensis were as follows：HPD100 Macroporous resin was fitted.The mass proportion of resin and medicine was 1 ∶ 1.74,the absorption capacity of HPD100 Macroporous resin was 0.15g/ml and the velocity of absorption was 2BV/h,the diameter-height ratio of resin column was 1∶ 10,the eluting reagent was 4BV water and 4BV 70％ ethanol,eluting rate was 1BV/h.Conclusion This optimized purification technology was convenient and feasible.It could suit industrialization production.