HPLC法测定两种乌头药材中生物碱的含量 |
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引用本文: | 柳文静 刘静涵. HPLC法测定两种乌头药材中生物碱的含量[J]. 西北药学杂志, 1998, 13(1): 8-9 |
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作者姓名: | 柳文静 刘静涵 |
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作者单位: | [1]中国大学药物分析教研室 [2]中国药科大学天然药化教研室 |
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摘 要: | 高效液相色谱法测定湖北两种乌头药材中乌头碱、次乌头碱、中乌头碱的含量。采用SpherisorbODS柱,以甲醇-水-氯仿-三乙胺(60:40:2:0.1)为流动相,联苯为内标。
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关 键 词: | 高效液相色谱法 乌头碱 中乌头碱 次乌头碱 |
Determination of main alkaloids in two species of Radix Aconiti by HPLC |
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Abstract: | An HPLC method was used to determinate the main alkaloids in two species of Radix Aconiti(Chuanwu and Caowu). Aconitine, mesaconitine, hypaconitine were separated on a Spherisob ODS column with biphenyl as internal standard substance. The mobile phase consisted of methanol, water, chloroform and triethylamine in ratio of 60: 40: 2: 0. 1. |
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Keywords: | HPLC aconitine mesaconitine hypaconitine |
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