散发性乳腺癌BRCA1基因的表达及其临床意义

目的　探讨BRCA1基因蛋白在汉族散发性乳腺癌中的表达及其临床病理意义。方法　采用免疫组化SP法对 5 1例乳腺癌石蜡切片组织进行BRCA1检测 ,并分析它与临床病理指标及c erbB 2表达的关系。　结果　BRCA1蛋白定位于癌细胞核 ,其失表达和低表达率为 49%。BRCA1蛋白表达下调与c erbB 2表达相关 (tr=4 15 5 ,P <0 0 5 ) ,与组织学分级、肿块大小、淋巴结转移数目及患者年龄均无明显相关。　结论　BRCA1蛋白表达的检测可在一定程度上反映基因的改变 ,可作为乳腺癌易感性初筛的手段。

BRCA1 protein expression in sporadic breast and its clinical significance

Objective To study BRCA1 gene expression in sporadic breast cancer in Han nationality and its relations to clinical pathological parameters. Methods Immunohistochemical method was used to detect the BRCA1 protein in paraffin embedded breast cancer tissue of 51 patients. The relationship between BRCA1 protein and clinical pathological features was analysed. Results BRCA1 protein was located in the nuclear of breast cancer cells. The rate of its down regulation of expression was 49.0% (25/51),which correlated reversely with c-erbB-2 protein,but did not correlate with histological grading,tumor size,lymph node involvement and patient age. Conclusion The detection of BRCA1 protein expression may be a reflection of its gene alteration. So it may be a primary method for screening the susceptibility to breast cancer.