血清凝集肿瘤细胞活性及其意义的研究

目的 :探讨血清对肿瘤细胞凝集的影响以及血清凝集肿瘤细胞活性测定的意义。方法 :采用体外细胞凝集实验和实验性转移模型等 ,以及以热变性、酶消化和盐析等方法处理血清。结果 :血清诱导促进高转移潜能肿瘤细胞凝集 ,以恶性肿瘤患者血清凝集肿瘤细胞活性最高 ,其对恶性肿瘤诊断的灵敏度为 90 6% ,特异性为92 1% ,准确性为 91 3%。糖肽能有效地抑制血清诱导的黑色素瘤B16 细胞凝集及其实验性肺转移。此外蛋白酶及高碘酸处理血清可使血清凝集肿瘤细胞活性极大的降低 ,正常人及良性瘤患者血清活性对热较稳定 ,而恶性肿瘤患者血清活性则比较敏感等。结论 :血清中存在肿瘤细胞凝集因子 ,正常人及良性瘤患者血清中因子可能主要是糖蛋白 ,恶性肿瘤患者血清中的则可能还有少量氨基多糖和凝集素类。血清可能通过诱导肿瘤细胞凝集促进肿瘤转移。此外 ,血清凝集肿瘤细胞活性与肿瘤转移相关 ,其在恶性肿瘤的临床诊断及预后方面可能具有重要的应用价值。

Study and evaluation on tumor cell agglutinating activity of human sera

Objective：To probe into the effect of human sera on tumor cell aggregation and evaluate tumor cell agglutinating activity of human sera.Methods：The aggregation of human nasopharyngeal carcinom a cells(CNE-2L2)with high metastatic potential induced by human sera in vitro and the experimental lung metastasis of B16 murine melanoma were quant ified.Human sera were treated by hot,pronase,salt out or periodate oxidation,etc .Results：L2 cell aggregatin was introduced and enhanced b y the sera of normal persons, benign tumor or malignant tumor patients,and the tumor cell aggregating activity of malignant tumor patients’ sera was the highe st.The diagnostic sensity to malignant tumor was 90.6%,the specificity was 92.1% and the accuracy was 91.3%.The experimental metastasis of B16 cells and t umor a ggregation were inhibited by laminin-glycopeptides.The activity of the sera tr eated by pronase-digestion and periodateoxidation was great reduced,however,the activity of both normal and benign sera was more stable to heat but the activit y of malignant sera was more sensitive. Conclusion: The study indicates that there are tumor cell aggreg ating factors in human sera,and it suggests that the factors in normal and benig n sera maybe mainly be glycoproteins,but the ones of malignant sera probably are glycoconjugates and lectins.These factors maybe enhance tumor cell embolization and metastasis by inducing tumor cell aggregation.Moreover it suggests that tum or cell agglutinating activity of human positively relate with tumor metastasis and may be a potential maker of malignant tumor and a valuable index in diagnosi s and prognosis of malignant tumor.