高效液相色谱法测定泰唑洗液中甲硝唑和醋酸氯己啶的含量

目的　建立泰唑洗液中甲硝唑和醋酸氯己啶含量的HPLC测定方法。方法　采用C18柱 (2 5 0mm× 4 .6mm ,10 μm ) ,流动相为甲醇 乙腈 三乙胺缓冲液 (0 .0 5mol·mL-1,用磷酸调节 pH至 2 .5 ) (30∶ 30∶ 4 0 ,v/v ) ,内标物为醋酸泼尼松。流速为 1.0mL·min-1,检测波长为 2 5 4nm。结果　线性范围 :甲硝唑 32～ 12 8μg·mL-1(r =0 .99996 ) ,醋酸氯己啶 2 0～80 μg·mL-1(r =0 .99997)。精密度 :日内及日间RSD为 :甲硝唑 0 .12 % (n =6 )和 0 .32 % (n =5 ) ;醋酸氯己啶 0 .18% (n =6 )和 0 .35 % (n =5 )。平均回收率甲硝唑为 10 0 .4 % (RSD =0 .19%n =5 ) ,醋酸氯己啶为 99.94 % (RSD= 0 .2 0 % ,n =5 )。结论　本法简便 ,快速 ,准确 ,可用于该制剂的质量控制

HPLC determination of metronidazole and chlorhexidine acetate in Taizou lotion

OBJECTIVE To establish the method on determination of metronidazole and chlorhexidine acetate in Taizou lotion by HPLC.METHOD An HPLC method was established by using a C18 column(250 mm ×4.6 mm , 10μm) as analytical column , methanol-acetonitrile-triethylamine buffer solution(0.05 mol*mL-1 ,adjusted pH to 2.5 with H3PO4)(30∶30∶40 ,v/v) as mobile phase , prednisone acetate as an internal standard . The flow rate was 1.0 mL*min-1, and detection wavelength was at 254 nm.RESULTS The linearity was obtained over the range of 32～128μg*mL-1 for metronidazole(r = 0.99996) and 20～80μg*mL-1 for chlorhexidine acetate(r = 0.99997) , respectively . The average recovery of method was 100.4% with RSD 0.19 % for metronidazole and 99.94% with RSD 0.20 % for chlorhexidine acetate , respectively. The intra-day and inter-day RSD for metronidazole were 0.12 %(n = 6) and 0.32 %(n = 5) , that for chlorhexidine acetate were 0.18 %(n = 6) and 0.35 %(n = 5) , respectively.CONCLUSIONS The method is simple , rapid , accurate and can be used for the quality control of the preparation.