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| 神经肽Y对培养的自发性高血压大鼠动脉平滑肌细胞的影响 | |
| 引用本文： | 吴翠环,任大宏,陈多恩. 神经肽Y对培养的自发性高血压大鼠动脉平滑肌细胞的影响[J]. 华中科技大学学报(医学版), 1998, 0(1) |
| 作者姓名： | 吴翠环 任大宏 陈多恩 |
| 作者单位： | 同济医科大学基础医学院病理学教研室!武汉430030 |
| 基金项目： | 国家自然科学基金资助项目(No.39370287) |
| 摘 要： | 应用细胞培养,~3(H-)胸腺嘧啶核苷和~3(H-)亮氨酸的掺入及细胞计数方法,研究缩血管物质神经肽Y(NPY)对血管平滑肌细胞生长的作用。结果发现,用神经肽Y作用48h后可促进离体培养的自发性高血压大鼠(SHR)动脉平滑肌细胞DNA合成增加28.0％,蛋白质合成增加54.6％,与对照组比较有显著性差异(P<0.01);而细胞数无明显增加(P>0.05)。提示,NPY对培养的SHR动脉平滑肌细胞有促肥大效应;SHR血管壁上NPY分布密度增加是引起高血压时血管壁结构改变的重要因素之一。 |
| 关 键 词： | 神经肽Y 自发性高血压 肌 平滑 肥大 |
Effect of Neuropeptide Y on the Cultured Arterial Smooth Muscle Cells in Spontaneously Hypertensive Rats |
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| Wu Cuihuan,Ren Dahong,Chen Duo'en. Effect of Neuropeptide Y on the Cultured Arterial Smooth Muscle Cells in Spontaneously Hypertensive Rats[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 1998, 0(1) | |
| Authors: | Wu Cuihuan Ren Dahong Chen Duo'en |
| Affiliation: | Wu Cuihuan,Ren Dahong,Chen Duo'en Department of Pathology,School of Basic Medical Sciences,Tongji Medical University,Wuhan 430030 |
| Abstract: | We applied the method of cellular culture, tritiated thymidine and leucine incorporation, and calculation in cell number to investigate the role of vasoconstrictor substance (neuropeptide Y NPY) in vascu-lar smooth muscle cell (VSMC) growth. It was observed that 48-hour exposure to neuropeptide Y for these cells caused an 28. 0 % and 54. 6 % increase respectively in DNA and protein synthesis in cultured VSMC in SHR (compared with serum control P<0, 01), but no significant increase in cell number (P>0. 05). The re-sults demonstrate that NPY accelerates VSMC hypertrophy in SHR, and an increase in density of NPY-con-taining innervation in the vasculature of SHR is one of the important factors in the structural changes of hy-pertensive vessels. |
| Keywords: | neuropeptide Y spontaneously hypertensive rats muscle smooth hypertrophy |
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