| 用双试剂与日立7170A分析仪测定血清无机磷 |
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| 引用本文： | 王断贵 蒋洪敏. 用双试剂与日立7170A分析仪测定血清无机磷[J]. 湖南医科大学学报, 1997, 22(5): 455-458 |
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| 作者姓名： | 王断贵 蒋洪敏 |
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| 摘 要： | 用日立７１７０Ａ分析仪以双试剂测定血清无机磷，并观察异常蛋白引起的干扰。该法线性范围可达５ｍｍｏｌ．Ｌ＾－１，准确度和精密度系数均在９５％以上，回收率在９８．２％￣９９．５％之间，脂浊血清及黄疸表对本法不产生干扰。双试剂与多发性骨髓瘤病人血清（含异常蛋白）不产生沉淀，是一种理想的血清无机磷测定法。
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| 关 键 词： | 磷酸盐类 无机磷 双试剂 血清 |
Determination of inorganic phosphate in serum by double reagents with Hitachi 7170 A analyzer] |
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| J Wang,H Jiang. Determination of inorganic phosphate in serum by double reagents with Hitachi 7170 A analyzer][J]. Bulletin of Hunan Medical University, 1997, 22(5): 455-458 |
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| Authors: | J Wang H Jiang |
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| Affiliation: | Department of Clinical Laboratory, Second Affiliated Hospital, Hunan Medical University, Changsha. |
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| Abstract: | We propose a method for determination of inorganic phosphate in serum using double reagents with Hitachi 7170 A analyzer. The standard curve for phosphate was linear from 1.0-5.0 mmol.L-1. Both coefficient of accuracy and precision were above 95%. The recovery rate ranged from 98.2%-99.5%. Jaundice Sample and lipohemia serum were found to be interference--free. This method can eliminate paraprotein interference, it was an ideal method for determination of inorganic phosphate in serum. |
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