联合检测568例乙型肝炎患者HBV DNA及Pre - S1Ag结果分析

目的 探讨检测乙型肝炎前S1蛋白及HBV DNA的临床意义.方法 采用ELISA法检测HBsAg、HBsAb、HBeAg、HBeAb、HBcAb、前S1蛋白;采用PCR法检测HBV DNA.结果 HBeAg阳性及阴性者Pre-SlAg的阳性率分别为83.7%和47.0%,HBsAg及HBeAg均是阳性者为88.5%;HBeAg阳性及阴性者HBV DNA阳性率分别为92.0%和49.7%,HBsAg及HBeAg均是阳性者为95.7%;Pre-S1Ag和HBV DNA的检测结果的符合率达90.8%,两者具有相关性(P＜0.001).结论 HBV DNA与乙型肝炎的复制状态极为密切,Pre-S1Ag测定在乙型肝炎的诊断及治疗过程中具有重要的临床价值,特别在不能检测HBV DNA的这些医院.

Analysis of HBV DNA plus PerS1Ag Detection in 568 Patients with Hepatitis B

Objective To evaluate the significance of detecting Pre-S 1 Ag and HBV DNA.Methods ELISA was used to detect HBsAg,HBsAb,HBeAg,HBeAb,HBcAb and Pre-SlAg.The HBV DNA was detected by PCR.Results The positive rate of Pre-SlAg in positive-HBeAg or negative-HBeAg patients was 83.7% and 47.0% respectively,but in positive-HBsAg and HBeAg patients it was 88.5%.The positiverate of HBV DNA in positive-HBeAg or negative-HBeAg patients was 92.0% and 49.7% respectively,butin positive-HBsAg and HgeAg patients it was 95.7%.The coincidence of Pre-SlAg and HBV DNA was 90.8%.There was correlation between Pre-SlAg and HBV DNA(P<0.001).Conclusion The relevance between HBV DNA and the degree of HBV duplication is more significant.The detection of Pre-S 1 Ag is more important in the diagnosis and therapy of HBV especially in hospital where HBV DNA cannot be detected.