匹罗卡品诱导癫痫大鼠内源性神经干细胞的可塑性改变

目的探讨匹罗卡品诱导癫痫模型的内源性神经干细胞的增殖、迁移,从而研究癫痫发生后的神经发生及可塑性改变.方法氯化锂和匹罗卡品联合诱导大鼠癫痫模型,分为对照组、癫痫发作后3、7、14、28 d组,用免疫组织化学方法动态检测大鼠海马多唾液酸神经细胞粘附分子(polysiolylated neural cell adhesion molecule,PSA-NCAM)及PSA-NCAM/5-溴脱氧尿苷嘧啶(bromodeoxyuridine,BrdU)的表达.结果与对照组比较,海马齿状回(dentate gyrus,DG)PSA-NCAM及PSA-NCAM/BrdU的阳性细胞在癫痫后3 d开始增加(P＜0.05),14 d达到高峰(P＜0.05),28 d后开始下降,但仍高于对照组(P＜0.05).结论癫痫发生后出现神经干细胞的增殖、迁移,也即出现了神经发生和可塑性改变.

Change of the plasticity of inherent neural stem cells in the epilepsy model induced by pilocarpine in rats

Objective To investigate the proliferation and migration of inherent neural stem cells in the epilepsy model induced by pilocarpine in rats.Methods The generalized seizure(GS) was induced by lithium-pilocarpine.The expression of polysiolylated neural cell adhesion molecule(PSA-NCAM) and PSA-NCAM/bromodeoxyuridine(BrdU) was observed in hippocampus by immunohisochemistry and immunofluorescence staining.Results Compared with the controls,the number of PSA-NCAM-positive cells and PSA-NCAM/BrdU positive cells in hippocampus increased at day 3 after GS(P<(0.05)),reached maximum at day 14(P<(0.01)),and decreased markedly at day 28,but it was still elevated compared with that of the controls(P<(0.05)).All positive cells in bilateral dentate gyrus(DG) were located in the deepest portion of the granule cell layter(GCL).Conclusions These observations indicate that GS may stimulate the proliferation and migration of inherent neural stem cells,as well as neurogenesis and plastic change of originally-existed neural stem cells may occur in response to the GS.